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The mitochondrial Ca2+ uniporter MCU is essential for glucose-induced ATP increases in pancreatic β-cells. | LitMetric

AI Article Synopsis

  • - Glucose stimulates insulin release from pancreatic β-cells via ATP production, membrane changes, and calcium influx, with recent research highlighting the role of mitochondrial calcium transport in this process.
  • - Using advanced imaging and electrophysiology techniques, the study demonstrates that the mitochondrial calcium uniporter (MCU) is essential for calcium uptake and maintaining ATP levels in response to glucose.
  • - Under stress conditions resembling diabetes, mitochondrial calcium uptake and ATP production are impaired, indicating that proper calcium handling by MCU and the sodium-calcium exchanger (NCLX) is critical for β-cell function.

Article Abstract

Glucose induces insulin release from pancreatic β-cells by stimulating ATP synthesis, membrane depolarisation and Ca(2+) influx. As well as activating ATP-consuming processes, cytosolic Ca(2+) increases may also potentiate mitochondrial ATP synthesis. Until recently, the ability to study the role of mitochondrial Ca(2+) transport in glucose-stimulated insulin secretion has been hindered by the absence of suitable approaches either to suppress Ca(2+) uptake into these organelles, or to examine the impact on β-cell excitability. Here, we have combined patch-clamp electrophysiology with simultaneous real-time imaging of compartmentalised changes in Ca(2+) and ATP/ADP ratio in single primary mouse β-cells, using recombinant targeted (Pericam or Perceval, respectively) as well as entrapped intracellular (Fura-Red), probes. Through shRNA-mediated silencing we show that the recently-identified mitochondrial Ca(2+) uniporter, MCU, is required for depolarisation-induced mitochondrial Ca(2+) increases, and for a sustained increase in cytosolic ATP/ADP ratio. By contrast, silencing of the mitochondrial Na(+)-Ca(2+) exchanger NCLX affected the kinetics of glucose-induced changes in, but not steady state values of, cytosolic ATP/ADP. Exposure to gluco-lipotoxic conditions delayed both mitochondrial Ca(2+) uptake and cytosolic ATP/ADP ratio increases without affecting the expression of either gene. Mitochondrial Ca(2+) accumulation, mediated by MCU and modulated by NCLX, is thus required for normal glucose sensing by pancreatic β-cells, and becomes defective in conditions mimicking the diabetic milieu.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3400633PMC
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0039722PLOS

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