Diversity of Streptomyces spp. in Eastern Himalayan region - computational RNomics approach to phylogeny.

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Microbiology Laboratory, Department of Biotechnology and Bioinformatics, North-Eastern Hill University, Shillong-793022.

Published: August 2012

Isolation and characterization of actinomycetes from soil samples from altitudinal gradient of North-East India were investigated for computational RNomics based phylogeny. A total of 52 diverse isolates of Streptomyces from the soil samples were isolated on four different media and from these 6 isolates were selected on the basis of cultural characteristics, microscopic and biochemical studies. Sequencing of 16S rDNA of the selected isolates identified them to belong to six different species of Streptomyces. The molecular morphometric and physico-kinetic analysis of 16S rRNA sequences were performed to predict the diversity of the genus. The computational RNomics study revealed the significance of the structural RNA based phylogenetic analysis in a relatively diverse group of Streptomyces.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3398779PMC
http://dx.doi.org/10.6026/97320630008548DOI Listing

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