The molecularly well-characterized auxin signal transduction pathway involves two evolutionarily conserved families interacting through their C-terminal domains III and IV: the Auxin Response Factors (ARFs) and their repressors the Aux/IAAs, to control auxin-responsive genes, among them genes involved in auxin transport. ( 1) (,) ( 2) We have developed a new genetic tool to study ARF function. Using MONOPTEROS (MP)/ARF5, we have generated a truncated version of MP (MPΔ), ( 3) which has lost the target domains for repression by Aux/IAA proteins. Besides exploring genetic interactions between MP and Aux/IAAs, we used this construct to trace MP's role in vascular patterning, a previously characterized auxin dependent process. ( 4) (,) ( 5) Here we summarize examples of naturally occurring truncated ARFs and summarize potential applications of truncated ARFs as analytical tools.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3474672 | PMC |
http://dx.doi.org/10.4161/psb.20366 | DOI Listing |
BMC Plant Biol
January 2025
Department of Biology, College of Science, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia.
Background: Samh (Mesembryanthemum forsskalii, M. cryptanthum) belongs to Aizoaceae family and is found in northern Saudi Arabia, primarily in desert or dry shrubland habitats. M.
View Article and Find Full Text PDFFront Plant Sci
December 2024
China Tobacco Gene Research Center (CTGRC), Zhengzhou Tobacco Research Institute of China National Tobacco Corporation (CNTC), Zhengzhou, China.
The INDETERMINATE DOMAIN (IDD) gene family, encoding a class of C2H2 transcription factor, played diverse roles in land plants. The IDD family in tobacco () has not been characterized. In this study, 26 NtIDDs were identified in the tobacco genome.
View Article and Find Full Text PDFMol Biol Rep
January 2025
Department of Biochemistry, Maharshi Dayanand University, Rohtak, 124001, India.
Plants frequently confront pathogens that disrupt physiological and molecular functions, ultimately reducing agricultural yields. To counter these challenges, plants activate sophisticated defense mechanisms to recognize stress signals while optimizing growth. Phytohormones signaling pathways and their crosstalk are central to regulating plant growth, development and defense.
View Article and Find Full Text PDFPhysiol Mol Biol Plants
December 2024
Department of Grassland Science, College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009 China.
Unlabelled: Auxin response factors (ARFs) are important transcription factors that regulate the expression of auxin response genes, thus play crucial roles in plant growth and development. However, the functions of genes in bermudagrass ( L.), a turfgrass species of great economic value, remain poorly understood.
View Article and Find Full Text PDFNat Commun
December 2024
Laboratory of Biochemistry, Wageningen University, Stippeneng 4, 6708WE, Wageningen, the Netherlands.
The Auxin Response Factors (ARFs) family of transcription factors are the central mediators of auxin-triggered transcriptional regulation. Functionally different classes of extant ARFs operate as antagonistic auxin-dependent and -independent regulators. While part of the evolutionary trajectory to the present auxin response functions has been reconstructed, it is unclear how ARFs emerged, and how early diversification led to functionally different proteins.
View Article and Find Full Text PDFEnter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!