AI Article Synopsis

  • The auxin signal transduction pathway is governed by the interaction of Auxin Response Factors (ARFs) and their repressors, the Aux/IAAs, which control auxin-responsive genes.
  • A new genetic tool has been developed to study ARF function by creating a truncated version of MONOPTEROS (MP), named MPΔ, that lacks domains for repression by Aux/IAAs.
  • The study highlights genetic interactions between MP and Aux/IAAs and further explores MP's role in vascular patterning, also noting the potential uses of truncated ARFs as analytical tools.

Article Abstract

The molecularly well-characterized auxin signal transduction pathway involves two evolutionarily conserved families interacting through their C-terminal domains III and IV: the Auxin Response Factors (ARFs) and their repressors the Aux/IAAs, to control auxin-responsive genes, among them genes involved in auxin transport. ( 1) (,) ( 2) We have developed a new genetic tool to study ARF function. Using MONOPTEROS (MP)/ARF5, we have generated a truncated version of MP (MPΔ), ( 3) which has lost the target domains for repression by Aux/IAA proteins. Besides exploring genetic interactions between MP and Aux/IAAs, we used this construct to trace MP's role in vascular patterning, a previously characterized auxin dependent process. ( 4) (,) ( 5) Here we summarize examples of naturally occurring truncated ARFs and summarize potential applications of truncated ARFs as analytical tools.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3474672PMC
http://dx.doi.org/10.4161/psb.20366DOI Listing

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