AI Article Synopsis
- SMA-Lipodisq nanoparticles were created with an average of one bacteriorhodopsin protein per 12 nm, using a protein/lipid ratio of 1:172 and without detergents.
- Electron paramagnetic resonance techniques showed that the structure and dynamics of bacteriorhodopsin were preserved in these nanoparticles compared to its native and detergent-based environments.
- This suggests that Lipodisq nanoparticles could serve as effective models for studying membrane proteins in a simulated environment.
Article Abstract
SMA-Lipodisq nanoparticles, with one bacteriorhodopsin (bR) per 12 nm particle on average (protein/lipid molar ratio, 1:172), were prepared without the use of detergents. Using pulsed and continuous wave nitroxide spin label electron paramagnetic resonance, the structural and dynamic integrity of bR was retained when compared with data for bR obtained in the native membrane and in detergents and then with crystal data. This indicates the potential of Lipodisq nanoparticles as a useful membrane mimetic.
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| http://dx.doi.org/10.1021/nl3020395 | DOI Listing |
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