Biomaterials are increasingly used for the restoration of human function, but can become infected as a result of peri- or early post-operative bacterial contamination, although biomaterial-associated infections (BAIs) can also initiate at any time from hematogenous spreading of bacteria from an infection elsewhere in the body. Infecting bacteria in BAIs not only seek shelter in their own protective biofilm matrix, but also hide in surrounding tissue. This study compares staphylococcal persistence on and around a degradable and non-degradable surgical mesh through the use of longitudinal bioluminescence imaging in a murine model, including histological evaluation of surrounding tissue after sacrifice. Surgical meshes were first contaminated with bioluminescent Staphylococcus aureus Xen29 and subsequently subcutaneously implanted in mice. Bioluminescent staphylococci persisted on and around non-degradable meshes during the 28-day course of the study, whereas bioluminescence returned to control levels and bacteria disappeared from surrounding tissues once a degradable mesh had fully dissolved. Thus the application of degradable biomaterials yields major advantages with respect to the prevention of BAIs, as dissolution of the implant not only is associated with elimination of the protective biofilm mode of growth of the infecting organisms, but also allows the immune system to clear the surrounding tissue from infecting organisms.
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http://dx.doi.org/10.1016/j.actbio.2012.07.017 | DOI Listing |
Objective: Aim: To identify cellular autophagy markers around nickel-containing implant as evidence of metal hypersensitivity reactions in an animal model.
Patients And Methods: Materials and Methods: Rats were sensitized to nickel using a modified model involving the administration of NiSO4 with adjuvants. Subsequently, nickel plate implants (Ni content at 98.
Int J Exp Pathol
December 2024
Department of Ageing, Rheumatology and Regenerative Medicine, Division of Medicine, The Rayne Building, University College, London, UK.
Using a model of UV-killed E. coli driven dermal inflammation in healthy human volunteers, we originally reported that following inflammatory resolution there was infiltration of macrophages, which, through prostanoids including prostaglandin (PG) E, imprints long-term tissue immunity. In addition to the prostanoids, data on levels of Specialised Pro-Resolution Lipid Mediators (SPMs) throughout inflammatory onset, resolution and post-resolution phases of this model were presented, but as illustrations rather than as primary data.
View Article and Find Full Text PDFAdv Mater
December 2024
Key laboratory of Biorheological Science and Technology, Ministry of Educations, College of Bioengineering, Chongqing University, Chongqing, 400044, China.
Intervertebral disc degeneration (IVDD) is characterized by fibrosis of nucleus pulposus (NP) cells and accelerated surrounding extracellular matrix catabolism. Bioactive hydrogels have shown significant potential in regulating cellular functions and tissue homeostasis. In this work, a dynamic hydrogel (HA-NCSN/Cu) is designed via the reductive chelation of hyaluronic acid grafted with thiourea (HA-NCSN) and Cu.
View Article and Find Full Text PDFExploration (Beijing)
December 2024
Paul C Lauterbur Research Center for Biomedical Imaging, Institute of Biomedical and Health Engineering Shenzhen Institute of Advanced Technology Chinese Academy of Sciences Shenzhen China.
Treating brain tumors requires a nuanced understanding of the brain, a vital and delicate organ. Location, size, tumor type, and surrounding tissue health are crucial in developing treatment plans. This review comprehensively summarizes various treatment options that are available or could be potentially available for brain tumors, including physical therapies (radiotherapy, ablation therapy, photodynamic therapy, tumor-treating field therapy, and cold atmospheric plasma therapy) and non-physical therapies (surgical resection, chemotherapy, targeted therapy, and immunotherapy).
View Article and Find Full Text PDFPlast Reconstr Surg Glob Open
December 2024
Department of Plastic, Reconstructive and Regenerative Surgery, Graduate School of Medicine, Nippon Medical School, Bunkyo Ward, Tokyo, Japan.
Background: Almost half of all spontaneously occurring keloids are acne keloids on the anterior chest. These keloids often grow in a crab-claw shape due to predominant tractional stresses on the scar; such stresses are risk factors for keloid growth/progression. To understand the relationship between acne keloid growth and mechanical stress, we conducted finite element analysis (FEA), measured the long/short dimensions of photographed acne keloids, and subjected acne keloids to microscopy.
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