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Efficacy of Molecular Methods for the Diagnosis of Enteropathogenic Microorganisms Associated with Diarrhoea: A Systematic Review.
J Gastrointestin Liver Dis
December 2024
Unidad Central del Valle del Cauca-UCEVA, Tuluá, Valle del Cauca, Colombia.
Background And Aims: Diarrhoea has a significant health impact and requires accurate diagnostic approaches, despite the limitations of many existing methods. This review examines various molecular techniques aimed at facilitating the rapid diagnosis of diarrhoeal diseases caused by bacterial, viral and parasitic pathogens.
Methods: A comprehensive systematic literature review was conducted using six prestigious databases, including WOS, Scopus, Science Direct, Embase, PubMed and LILACS Plus.
Frequency of Bovine Respiratory Disease Complex Bacterial and Viral Agents Using Multiplex Real-Time qPCR in Quebec, Canada, from 2019 to 2023.
Vet Sci
December 2024
Biovet Inc., Division of Antech Diagnostics and Mars Petcare Science & Diagnostics Company, Saint-Hyacinthe, QC J2S 8W2, Canada.
The bovine respiratory disease complex (BRD) is a multifactorial disease caused by various bacterial and viral pathogens. Using rapid pathogen detection techniques is helpful for tailoring therapeutic and preventive strategies in affected animals and herds. The objective of this study was to report the frequency of 10 pathogens by multiplex RT-qPCR on samples submitted for BRD diagnosis to a diagnostic laboratory (Biovet Inc.
View Article and Find Full Text PDFDevelopment of a triplex crystal digital RT-PCR for the detection of PHEV, PRV, and CSFV.
Front Vet Sci
December 2024
College of Animal Science and Technology, Guangxi University, Nanning, China.
Porcine hemagglutinating encephalomyelitis virus (PHEV), porcine pseudorabies virus (PRV), and classical swine fever virus (CSFV) are currently prevalent worldwide and cause similar neurological symptoms in infected pigs. It is very important to establish a detection method that can rapidly and accurately detect and differentiate these three viruses. Targeting the PHEV N gene, PRV gB gene, and CSFV 5' untranslated region (5'UTR), three pairs of specific primers and probes were designed, and a triplex crystal digital reverse transcription-PCR (cdRT-PCR) was developed to detect PHEV, PRV, and CSFV.
View Article and Find Full Text PDFMolecular surveillance of Plasmodium falciparum histidine-rich protein 2/3 gene deletions in Mozambique, 2023.
Malar J
December 2024
Centro de Investigação em Saúde de Manhiça, Maputo, Mozambique.
Background: Rapid diagnostic tests (RDTs) based on the detection of Plasmodium falciparum histidine rich protein 2 (PfHRP2) are widely used for the diagnostic of P. falciparum in Africa. However, deletions of the pfhrp2 and pfhrp3 genes can lead to false negative test results and compromise appropriate case management.
View Article and Find Full Text PDFSensitive and accurate proteome profiling of embryogenesis using Real-Time Search and TMTproC quantification.
Mol Cell Proteomics
December 2024
Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, United States; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, United States; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, United States. Electronic address:
Multiplexed proteomics has become a powerful tool for investigating biological systems. Using balancer-peptide conjugates (e.g.
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