Background: There are several reports describing thousands of SSR markers in the peanut (Arachis hypogaea L.) genome. There is a need to integrate various research reports of peanut DNA polymorphism into a single platform. Further, because of lack of uniformity in the labeling of these markers across the publications, there is some confusion on the identities of many markers. We describe below an effort to develop a central comprehensive database of polymorphic SSR markers in peanut.
Findings: We compiled 1,343 SSR markers as detecting polymorphism (14.5%) within a total of 9,274 markers. Amongst all polymorphic SSRs examined, we found that AG motif (36.5%) was the most abundant followed by AAG (12.1%), AAT (10.9%), and AT (10.3%).The mean length of SSR repeats in dinucleotide SSRs was significantly longer than that in trinucleotide SSRs. Dinucleotide SSRs showed higher polymorphism frequency for genomic SSRs when compared to trinucleotide SSRs, while for EST-SSRs, the frequency of polymorphic SSRs was higher in trinucleotide SSRs than in dinucleotide SSRs. The correlation of the length of SSR and the frequency of polymorphism revealed that the frequency of polymorphism was decreased as motif repeat number increased.
Conclusions: The assembled polymorphic SSRs would enhance the density of the existing genetic maps of peanut, which could also be a useful source of DNA markers suitable for high-throughput QTL mapping and marker-assisted selection in peanut improvement and thus would be of value to breeders.
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http://dx.doi.org/10.1186/1756-0500-5-362 | DOI Listing |
PeerJ
January 2025
Guangxi Key Laboratory of Efficacy Study on Chinese Materia Medica, Nanning, Guangxi, China.
Background: var. is a variety in the section of the genus of the family Theaceae which is native to Fangchenggang, Guangxi, China. To date, the genetic diversity and structure of this variety remains to be understood.
View Article and Find Full Text PDFPlants (Basel)
January 2025
Maize Research Institute Zemun Polje, Slobodana Bajića 1, 11185 Belgrade, Serbia.
Driven by the growing demands for plant-based protein in Europe and attempts of soybean breeding programs to improve the productivity of created varieties, this study aimed to enhance genetic resource utilization efficiency by providing information relevant to well-focused breeding targets. A set of 90 accessions was subjected to a comprehensive assessment of genetic diversity in a soybean working collection using three marker types: morphological descriptors, agronomic traits, and SSRs. Genotype grouping patterns varied among the markers, displaying the best congruence with pedigree data and maturity for SSRs and agronomic traits, respectively.
View Article and Find Full Text PDFPlants (Basel)
January 2025
Department of Soil, Plant and Food Sciences (DiSSPA), University of Bari-Aldo Moro, Via Giovanni Amendola 165/A, 70126 Bari, Italy.
subsp. (), a quarantine pathogen in the European Union, severely threatens Mediterranean olive production, especially in southern Italy, where Olive Quick Decline Syndrome (OQDS) has devastated Apulian olive groves. This study addresses the urgent need to identify resistant olive genotypes by monitoring 16 potentially tolerant genotypes over six years, assessing symptom severity and bacterial load.
View Article and Find Full Text PDFGenes (Basel)
January 2025
Floriculture Research Division, National Institute of Horticultural & Herbal Science, Rural Development Administration, Wanju 55365, Republic of Korea.
Background/objectives: Chrysanthemum (), a key ornamental and medicinal plant, presents challenges in cultivar identification due to high phenotypic similarity and environmental influences. This study assessed the genetic diversity and discrimination of 126 spray-type chrysanthemum cultivars.
Methods: About twenty-three simple sequence repeat (SSR) markers were screened for the discrimination of 126 cultivars, among which six SSR markers showed polymorphic fragments.
Genes (Basel)
January 2025
State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China.
Background/objectives: The Pacific abalone originated in cold waters and is an economically important aquaculture shellfish in China. Our goal was to clarify the current status of the genetic structure of Pacific abalone in China.
Methods: In this study, eighteen polymorphic EST-SSR loci were successfully developed based on the hemolymph transcriptome data of Pacific abalone, and thirteen highly polymorphic EST-SSR loci were selected for the genetic variation analysis of the six populations collected.
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