Background: Resistance to glyphosate in weed species is a major challenge for the sustainability of glyphosate use in crop and non-crop systems. A glyphosate-resistant Italian ryegrass population has been identified in Arkansas. This research was conducted to elucidate its resistance mechanism.
Results: The investigation was conducted on resistant and susceptible plants from a population in Desha County, Arkansas (Des03). The amounts of glyphosate that caused 50% overall visual injury were 7 to 13 times greater than those for susceptible plants from the same population. The EPSPS gene did not contain any point mutation that has previously been associated with resistance to glyphosate, nor were there any other mutations on the EPSPS gene unique to the Des03 resistant plants. The resistant plants had 6-fold higher basal EPSPS enzyme activities than the susceptible plants, but their I(50) values in response to glyphosate were similar. The resistant plants contained up to 25 more copies of EPSPS gene than the susceptible plants. The level of resistance to glyphosate correlated with increases in EPSPS enzyme activity and EPSPS copy number.
Conclusion: Increased EPSPS gene amplification and EPSPS enzyme activity confer resistance to glyphosate in the Des03 population. This is the first report of EPSPS gene amplification in glyphosate-resistant Italian ryegrass. Other resistance mechanism(s) may also be involved.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/ps.3342 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Increase in IAA levels by EPSPS copy number variation relates to fitness advantage in Eleusine indica.
Pest Manag Sci
January 2025
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Background: Long-term use of chemical weed control has led to some weedy species evolving herbicide resistance traits with fitness advantage. Our previous studies revealed glyphosate resistance in an Eleusine indica population due to copy number variation of 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) comes with fitness advantage under non-competitive conditions. Here, transcriptomics and targeted metabolomics were used to investigate physiological basis associated with the fitness advantage.
View Article and Find Full Text PDFAffecting of Glyphosate Tolerance and Metabolite Content in Transgenic Overexpressing Gene from .
Plants (Basel)
December 2024
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Long-term use of the global non-selective herbicide glyphosate for weed control has caused resistance in weeds. Overproducing of the target of glyphosate 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) is one of the resistance mechanisms in weeds. However, few studies have measured the effects on tolerance levels and metabolite content in model plant species overexpressing from weeds.
View Article and Find Full Text PDFHighly Efficient Mediated Transformation of Oil Palm Using an -Glyphosate Selection System.
Plants (Basel)
November 2024
National Key Laboratory for Tropical Crop Breeding, College of Tropical Agriculture and Forestry, Hainan University, Sanya 572025, China.
Oil palm ( Jacq.) is the most efficient oil-producing crop globally, yet progress in its research has been hampered by the lack of effective genetic transformation systems. The gene, encoding 5-enolpyruvylshikimate-3-phosphate synthase, has been used as a transgenic selection marker in various crops, including rice and soybean.
View Article and Find Full Text PDFUnraveling the mechanisms of multiple resistance across glyphosate and glufosinate in Eleusine indica.
Pestic Biochem Physiol
December 2024
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address:
The herbicides glyphosate and glufosinate are commonly used in citrus and sugarcane orchards in Guangxi Province, China, wherein the C plant Eleusine indica (L.) Gaertn. is known to be a dominant weed species.
View Article and Find Full Text PDFCytogenetic characterization of EPSPS gene amplification in glyphosate-resistant Hordeum glaucum and Bromus diandrus from Australia.
Plant J
December 2024
Department of Agronomy, Kansas State University, Manhattan, Kansas, USA.
Article Synopsis
- Two polyploid grass weeds in Australia, Hordeum glaucum and Bromus diandrus, have developed resistance to glyphosate through the amplification of the EPSPS gene, which is crucial for their survival.
- Research involved analyzing the EPSPS gene's genomic structure using molecular cytogenetic methods, showing that resistant plants have significantly more copies of the gene compared to susceptible ones.
- The findings suggest that unequal crossover during meiosis may be responsible for the gene duplication, contributing to the evolution of glyphosate resistance in these weed species.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!