Proteomic analysis of the human body is a significant recent scientific endeavour. In this study, we investigated the proteomic profile of human dentin using modern analytical and mass spectrometric techniques. Five healthy permanent human molars from five adults were cut, pulverized, denaturated with guanidine buffer, and demineralized with EDTA buffer. The extracted proteins were analysed by gel electrophoresis (SDS-PAGE and two-dimensional gel electrophoresis), digested with trypsin, and separated by liquid chromatography/high-resolution tandem mass spectrometry. We identified 289 proteins with high confidence, 90 of which had not been previously detected in human dentin. Nine (currently hypothetical) proteins were identified for the first time in an actual human sample. The proteins have a variety of functions, including calcium-ion binding, formation of the extracellular matrix, formation of the cytoskeleton, cytoskeletal protein binding, immune response, and transport. In conclusion, this is the first use of two-dimensional electrophoresis for investigating human dentin.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1600-0722.2012.00977.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Comparative Study of Anti-COVID Mouthwash and Remineralization Agents on Dentinal Tubular Occlusion: An Study.
J Int Soc Prev Community Dent
December 2024
Assistant professor, Oral and Dental Disease Research Center, Department of Operative Dentistry, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran.
Aim: Tooth sensitivity caused by exposed dentin tubules is a common clinical problem requiring correct treatment methods. Owing to the spread of the COVID-19 virus, it has become common to use different mouthwashes, including 1.5% hydrogen peroxide (HP), before dental procedures.
View Article and Find Full Text PDFOsteopontin Facilitated Dental Pulp Cell Adhesion and Differentiation: A Laboratory Investigation.
ACS Appl Bio Mater
January 2025
The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing 210008, China.
Aim: To investigate the effects of osteopontin (OPN) on cultured human dental pulp cells (hDPCs) in relation to adhesion, proliferation, differentiation, and mineralization.
Methodology: Subcultured hDPCs isolated from healthy human wisdom teeth were inoculated on noncoated (NC, control) and OPN-coated nontissue culture-treated polystyrene plates (Non-TCPS). Cell adhesion and proliferation were analyzed by crystal violet staining and the CCK-8 assay, respectively.
The influence of tooth shade on near-infrared light transmission through human dentine and enamel: an in-vitro study.
Lasers Med Sci
January 2025
School of Medicine and Dentistry, Griffith University, Gold Coast, QLD, Australia.
This in-vitro study assessed the influence of the shade of human teeth on the transmission of near-infrared light. A total of 40 teeth were used. After cleaning the root surface and removing cementum, the teeth were sectioned into slices 3 mm thick, with each comprising a portion of the crown (enamel-dentine (ED)) and of the root (dentine only).
View Article and Find Full Text PDFEvaluation of Biodentine® and Calcium Hydroxide in the Formation of Dentin Bridge in Deep Carious Lesions.
West Afr J Med
September 2024
.Department of Preventive Dentistry, Lagos State University, College of Medicine, Faculty of Dentistry, Ikeja, Lagos, PMB 21266, Nigeria.
Background: Indirect pulp capping is the main treatment modality for reversible pulpitis.
Objective: To evaluate the efficacy of Biodentine® and Calcium hydroxide in the formation of dentin bridge.
Materials And Methods: A double blinded, randomized clinical control trial involving 50 consenting subjects, aged 16 to 55 years with deep carious vital teeth.
Are ultrasonic tips associated with final irrigation protocols effective in removing biofilms in long oval canals and dentinal tubules?
Clin Oral Investig
January 2025
Department of Restorative Dentistry, Dental Materials, and Endodontics, Bauru School of Dentistry, University of São Paulo, Rua Siqueira Campos, 180, Centro, Vitória da Conquista, Bauru, São Paulo, BA, ZIP: 45.000-455, Brazil.
Objective: This study investigated the associations among endodontic instruments, ultrasonic tips and various final irrigation protocols for removing intracanal and intratubular biofilms in long oval canals.
Methodology: One hundred mandibular premolars inoculated with Enterococcus faecalis were divided into two groups: the control group (CG: n = 10), which received no treatment; and the test groups (n = 30), which included saline (SS), sodium hypochlorite (2.5% NaOCl) and chlorhexidine (2% CHX).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!