Objective: To explore the value of flow cytometry in anaphylactic shock diagnosis by CD63 expression being detected using flow cytometry to conform the activation of basophils.
Methods: Sixteen rats were randomly divided into two groups: control group and anaphylactic shock group. The model of anaphylactic shock rat with ovalbumin injection was established. CD63, CD45 and CD203c antibody combination, flow cytometry was employed to detected blood basophil CD63 expression. Immunofluorescence method was employed to observe the CD63 immunofluorescence staining in the rat lung tissue.
Results: (1) Pure basophils were obtained by CD45 and CD203c gating. (2) The percentages of basophils CD63 were (17.34 +/- 2.04)% and (1.52 +/- 0.35)% in the experimental and control group, respectively. The differences between two groups were statistically significant (P < 0.01). (3) Compared with the control group, the expression of CD63 in basophils increased in anaphylactic shock lung tissue.
Conclusion: The detection of CD63 by flow cytometry could be the supplement of vivo allergic reactions and have good clinical value.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
The development of murine bone marrow-derived mast cells expressing functional human MRGPRX2 for and studies.
Front Immunol
January 2025
Department of Basic and Translational Sciences, School of Dental Medicine, University of Pennsylvania, Philadelphia, PA, United States.
Introduction: A subtype of human mast cells (MCs) found in the skin and to a lesser extent in the lung and gut express a novel G protein-coupled receptor (GPCR) known as Mas-related GPCR-X2 (MRGPRX2, mouse counterpart MrgprB2). In addition to drug-induced pseudoallergy and cutaneous disorders, MrgprB2 contributes to ulcerative colitis, IgE-mediated lung inflammation and systemic anaphylaxis. Interestingly, most agonists activate MRGPRX2 with higher potency than MrgprB2.
View Article and Find Full Text PDFParents' practices, feelings, and support needs surrounding the development of food allergy literacy (FAL) in children before their transition to school.
Heliyon
November 2024
Allergy & Anaphylaxis Australia, Australia.
Children's knowledge of food allergy and risk prevention, coined as food allergy literacy (FAL), can bolster their safety in schools. Addressing research gaps on this topic, especially, parents' role, the aim of this study was to investigate Australian parents' practices, feelings, and support needs relating to building children's FAL before their transition to school. Questionnaire data from 159 parents were analysed statistically to characterise the sample and explore the association between children's FAL, parent anxiety, and parent confidence in their child's ability to stay safe at school.
View Article and Find Full Text PDFEpicutaneous immunotherapy for food allergy: a systematic review and meta-analysis.
Syst Rev
January 2025
Department of Pediatric, Affiliated Chifeng Clinical College of Inner, Mongolia Medical University, Chifeng, China.
Background: There is ongoing debate about the safety and efficacy of epicutaneous immunotherapy (EPIT) in treating food allergies. The systematic review and meta-analysis aimed to evaluate the safety and efficacy of EPIT.
Methods: We systematically searched international trial registers (ClinicalTrials.
Opioid Allergy Cross-Reactivity: A Retrospective Study Across Three Opioid Classes.
J Pain Palliat Care Pharmacother
January 2025
Lexington VA Health Care System, Lexington, Kentucky, USA.
IgE-mediated opioid hypersensitivities, or true allergies, are rare and most adverse reactions to opioids can be attributed to side effects or to pseudo-allergies. Given that immune-mediated allergies to opioids are uncommon, literature regarding cross-reactivity among opioid classes are limited. This retrospective study aimed to determine the rates of cross-reactivity and tolerance among patients with previously documented opioid allergy or adverse drug reaction (ADR) across three opioid drug classes (natural, semisynthetic, and synthetic opioids).
View Article and Find Full Text PDFEarly markers of baked milk and egg tolerance in young children with IgE-mediated immediate reactions.
Eur Ann Allergy Clin Immunol
January 2025
Division of Allergy and Immunology, Department of Pediatrics, Marmara University, School of Medicine, Istanbul, Turkey.
Children with milk and egg allergies have outcomes in which, three-quarters are tolerant to baked forms of the allergenic food. Identifying predictors of tolerance to baked foods for IgE-mediated immediate-type reactions may guide the early introduction of baked allergens to diet and tolerance development. This study explores factors associated with early tolerance to baked foods.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!