Fermentation-derived products are in greater demand to meet the increasing global market as well as to overcome environmental problems. In this work, Escherichia coli has been metabolically engineered with acrylate pathway genes from Clostridium propionicum for the conversion of D-lactic acid to propionic acid. The introduced synthetic pathway consisted of seven genes encoding the enzymes propionate CoA-transferase (Pct), lactoyl-CoA dehydratase (Lcd) and acryloyl-CoA reductase (Acr). The engineered strain synthesised propionic acid at a concentration of 3.7 ± 0.2 mM upon fermentation on glucose. This low production level could be attributed to the low activity of the recombinant enzymes in particular the rate-limiting enzyme, Acr. Interestingly, the recombinant pathway caused an increased lactate production in E. coli with a yield of 1.9 mol/mol of glucose consumed along with a decrease in other by-products. Down-regulation of the pfl (pyruvate formate lyase) genes and a possible inhibition of Pfl activity by the acrylate pathway intermediate, acryloyl-CoA, could have reduced carbon flow to the Pfl pathway with a concomitant increase in lactate production. This study reports a novel way of synthesising propionic acid by employing a non-native, user-friendly organism through metabolic engineering.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00253-012-4274-y | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Basic Science and Pathogenesis.
Alzheimers Dement
December 2024
The Ohio State University, Columbus, OH, USA.
Background: Microglia, the innate immune cells of the brain, are a principal player in Alzheimer's Disease (AD) pathogenesis. Their surveillance of the brain leads to interaction with the protein aggregates that drive AD pathogenesis, most notably Amyloid Beta (Aβ). Aβ can elicit attempts from microglia to clear and degrade it using phagocytic machinery, spurring damaging neuroinflammation in the process.
View Article and Find Full Text PDFExamination of the Bioavailability and Bioconversion of Wheat Bran-Bound Ferulic Acid: Insights into Gastrointestinal Processing and Colonic Metabolites.
J Agric Food Chem
January 2025
School of Food Science and Engineering, Wuhan Polytechnic University, Wuhan, 430023, China.
The postingestion journey and bioconversion of wheat bran-bound ferulic acid, a known beneficial phytochemical, remain insufficiently understood. This study aims to systematically investigate its bioaccessibility, bioavailability, excretion, and colonic metabolism, both and . Initial analysis confirmed the abundance and bioactivity of ferulic acid in wheat bran.
View Article and Find Full Text PDFDietary Methionine Restriction Alleviates Cognitive Impairment in Alzheimer's Disease Mice via Sex-Dependent Modulation on Gut Microbiota and Tryptophan Metabolism: A Multiomics Analysis.
J Agric Food Chem
January 2025
Laboratory of Functional Chemistry and Nutrition of Food, College of Food Science and Engineering, Northwest A&F University, Yangling, Shaanxi 712100, China.
Plant-based foods with low methionine contents have gained increasing interest for their potential health benefits, including neuroprotective effects. Methionine restriction (MR) linked to a plant-based diet has been shown to mitigate neurodegenerative diseases such as Alzheimer's disease (AD) through mechanisms that involve the gut microbiota. In this study, a 16-week MR diet (0.
View Article and Find Full Text PDFThe direct and indirect inhibition of proinflammatory adipose tissue macrophages by acarbose in diet-induced obesity.
Cell Rep Med
December 2024
Medical Research Center, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China; Department of Gastroenterology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing 100020, China; Immunology Research Center for Oral and Systemic Health, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; General Surgery Department, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China; Beijing Laboratory of Oral Health, Capital Medical University School of Basic Medicine, Beijing 100069, China; Beijing Key Laboratory of Tolerance Induction and Organ Protection in Transplantation, Beijing Friendship Hospital, Beijing 100050, China. Electronic address:
Inflammation is critical for obesity and obesity-induced insulin resistance (IR). In this study, we reveal the function and mechanism of acarbose on adipose tissue macrophage (ATM)-mediated inflammation in obesity and obesity-induced IR. First, acarbose enhances the abundance of propionic acid-producing Parasutterella, therefore indirectly inhibiting the survival and proinflammatory function of M1-like ATMs via GPR43.
View Article and Find Full Text PDFFacile and Highly Selective Deprotection of Aryl Propionates/Acetates Using a Supported Lewis Acid Catalyst (20% InCl/MCM-41).
ACS Omega
December 2024
Heterogeneous Catalysis Group, Department of Chemistry, Progressive Education Society's, Modern College of Arts, Science and Commerce (Autonomous) Shivajinagar, Pune 5, Maharashtra 411005, India.
The selective deprotection of substituted aryl esters like acetates and propionates in the presence of different electron-donating and -withdrawing functional groups to the corresponding phenols in good yields was reported using the Lewis acid supported solid acid catalyst 20 InCl/MCM-41 prepared by the wet impregnation method. The textural and microscopic properties of the catalyst were studied, which revealed a high degree of dispersion of InCl over MCM-41, promising quantification of Lewis acidity, and well-ordered honeycomb structure. The methodology was further explored for the selective deprotection of acetates and propionates in the presence of substituted amides that remain unaltered.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!