A rapid high-performance liquid chromatography method was developed to determine hippurate hydrolysis by Legionella spp. Benzoic acid, an end product of enzymatic activity, was directly detected by high-performance liquid chromatography after 1 and 24 h of incubation in 1% sodium hippurate. Because of its sensitivity, this procedure offers more precise identification of some Legionella spp.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC268287 | PMC |
| http://dx.doi.org/10.1128/jcm.28.12.2828-2829.1990 | DOI Listing |
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