AI Article Synopsis
- The study investigates the impact of dietary poisoning with aristolochic acids (AA) on Endemic Nephropathy (EN) and related cancers, revealing significant changes in the urinary proteome of AA-treated mice.
- Researchers utilized a mixed method of gel-based and in-solution tryptic digestion alongside LC-ESI-MS/MS to analyze the proteomes from urine, plasma, and kidney tissue.
- Findings identified key proteins linked to kidney health and inflammation that may serve as potential biomarkers for EN and associated urinary tract cancers in humans.
Article Abstract
Strong indications have been presented that dietary poisoning with aristolochic acids (AA) is responsible for Endemic Nephropathy (EN) and AA associated cancer of the upper urinary tract (UUTC). Our recent investigation showed drastic urinary proteome changes in AA treated mice. This study was designed to identify proteome changes associated with AA nephrotoxicity in experimental animal model. The DBA and C57BL mice, which differ in AA sensitivity, were exposed to AA for 4 days. The strategy for urinary, plasma and kidney tissue proteome study of AA exposed and control mice integrated gel-based and in-solution tryptic digestion combined with LC-ESI-MS/MS. To maximize proteome coverage, plasma fractionation scheme was developed and MS compatible sequential tissue extraction procedure was established. Proteomic analyses of urinary, plasma and kidney tissue tryptic digests resulted in identification of several cytoskeletal proteins, as well as proteins involved in kidney development and inflammatory response, that are differentially expressed in both AA exposed and control mice. These proteins are consistent with renal pathogenesis of endotoxicity and cancer. This proteomic strategy could be effectively translated for unbiased discovery of potential biomarkers for EN and associated UUTC in humans. At the same time, these results highlight the significance of AA exposure with EN. This article is part of a Special Issue entitled: Integrated omics.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.jprot.2012.06.026 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Absorptivity Is an Important Determinant in the Toxicity Difference between Aristolochic Acid I and Aristolochic Acid II.
J Agric Food Chem
January 2025
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.
Inadvertent exposure to aristolochic acids (AAs) is causing chronic renal disease worldwide, with aristolochic acid I (AA-I) identified as the primary toxic agent. This study employed chemical methods to investigate the mechanisms underlying the nephrotoxicity and carcinogenicity of AA-I. Aristolochic acid II (AA-II), which has a structure similar to that of AA-I, was investigated with the same methods for comparison.
View Article and Find Full Text PDFElucidation of the Active Agents in a West African Ground Herbal Medicine Formulation That Elicit Antimalarial Activities in In Vitro and In Vivo Models.
Molecules
November 2024
School of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA 30332, USA.
Agunmu (ground herbal medicine) is a form of West African traditional medicine consisting of a cocktail of herbs. The goal of this study is to evaluate a formulation of Agunmu made from , , , , and , sold in the open market and commonly used for the treatment of malaria by the locals, for its antimalarial effects and to determine the active principles that may contribute to the antimalarial effect. The ethanolic extract obtained from this formulation (Ag-Iba) was analyzed, using TLC, LC-MS, and Tandem-MS techniques, to determine its phytochemical properties.
View Article and Find Full Text PDFIn vitro nephrotoxicity and structure-toxicity relationships of eight natural aristolactams.
Toxicon
January 2025
Department of Natural Medicine, School of Pharmacy, Fudan University, Shanghai, 201203, China. Electronic address:
Article Synopsis
- - The study investigates the nephrotoxic effects of eight natural aristolactams (ALs) on HK-2 human kidney cells, highlighting their structural similarities to aristolochic acids (AAs), which raises safety concerns due to wider distribution of ALs in plants.
- - Results from MTT and ELISA assays indicate that all tested ALs exhibit nephrotoxicity, with AL Ⅰ, AL BⅡ, and velutinam showing the strongest cytotoxic effects, while structure-toxicity relationships reveal key functional groups influencing their toxicity levels.
- - The study found that the most harmful ALs not only cause increased kidney injury marker levels (KIM-1) and oxidative stress but also promote fibrosis
Berberine-based hypercrosslinked polymer: Advanced solid-phase microextraction for high-throughput analysis of aristolochic acids in environmental water and herbal plants.
Talanta
December 2024
Academy of Chinese Medical Science, Henan University of Chinese Medicine, Zhengzhou 450046, China; Collaborative Innovation Center of Research and Development on the Whole Industry Chain of Yu-Yao, Henan Province, China. Electronic address:
In this study, a novel berberine based anion-exchanged hypercrosslinked polymer (BBR-HCPs) was synthesized via Friedel-Crafts alkylation using dimethoxymethane as a cross-linking agent. The proposed BBR-HCPs polymer exhibited excellent adsorption capacities for aristolochic acids (AAs). Moreover, there are various interactions such as hydrophobic, π-π stacking, hydrogen bonding, and electrostatic interactions between BBR-HCPs and AAs.
View Article and Find Full Text PDFAristolochic Acids and Aristoloxazines Are Widespread in the Soil of Aristolochiaceae Herb Cultivation Fields.
Environ Sci Technol
December 2024
Department of Chemistry, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon 999077, Hong Kong, China.
The cancer risk associated with aristolochic acid (AA) exposure through the consumption of AA-containing herbal medicine has received tremendous attention in the past decades. However, environmental exposure routes from the associated medicinal herb cultivation fields have received little attention. We reveal through liquid chromatography-tandem mass spectrometry analysis of over 400 soil samples collected from three different Aristolochiaceae herb cultivation fields that AAs, which are nephrotoxic and carcinogenic, and aristoloxazines (AXs), a family of recently identified neurotoxic and genotoxic AA analogues, are widespread pollutants in these areas.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!