The genera of Mallotus and Phyllanthus contain several species that are commonly used as traditional medicines in oriental countries. Some species show interesting pharmaceutical activities, such as an antioxidant activity. To produce clinically useful medicines or food supplements (nutraceuticals) from these herbs, the species should be identified and a thorough quality control should be implemented. Nowadays, the integration of chromatographic and chemometric approaches allows a high-throughput identification and activity prediction of medicinal plants. In this study, Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were applied and compared to distinguish Mallotus and Phyllanthus species. Moreover, peaks from their chromatographic fingerprints, which were responsible for their antioxidant activity were assigned. For the latter purpose, the relevant information was extracted from the chromatographic fingerprints using linear multivariate calibration techniques, i.e., Partial Least Squares (PLS) and Orthogonal Projections to Latent Structures (O-PLS). Results reveal that exploratory analysis using PCA shows somewhat diverging clustering tendencies between Mallotus and Phyllanthus samples than HCA. However, both approaches mainly confirm each other. Concerning the multivariate calibration techniques, both PLS and O-PLS models demonstrate good predictive abilities. By comparing the regression coefficients of the models with the chromatographic fingerprints, the peaks that are potentially responsible for the antioxidant activity of the extracts could be confirmed.
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http://dx.doi.org/10.1016/j.jchromb.2012.06.025 | DOI Listing |
Trop Anim Health Prod
January 2021
Ecole des Sciences et Techniques de Production Animale Faculté des Sciences Agronomiques, Université d'Abomey-Calavi, 03 BP 2819, Cotonou, Benin.
In order to identify the most promising browse species for sustaining goat production, the nutritional characteristics of leaves of trees and shrubs and their use in farmers' feeding strategies were assessed in sub-humid areas of Benin. Two hundred and forty (240) goat farmers were surveyed and their uses of different leaves of trees and shrubs for supplementing their goats documented in two vegetation zones, namely the Sudano-Guinean and Guineo-Congolese zones. Then, samples of 32 leaves were collected at the end of rainy season in October-November, just before the long dry season and analyzed for their chemical composition, in vitro digestibility, and tannin content.
View Article and Find Full Text PDFAnal Chim Acta
June 2015
Department of Analytical Chemistry and Pharmaceutical Technology (FABI), Center for Pharmaceutical Research (CePhaR), Vrije Universiteit Brussel (VUB), Laarbeeklaan 103, B-1090 Brussels, Belgium. Electronic address:
Mallotus and Phyllanthus genera, both containing several species commonly used as traditional medicines around the world, are the subjects of this discrimination and classification study. The objective of this study was to compare different discrimination and classification techniques to distinguish the two genera (Mallotus and Phyllanthus) on the one hand, and the six species (Mallotus apelta, Mallotus paniculatus, Phyllanthus emblica, Phyllanthus reticulatus, Phyllanthus urinaria L. and Phyllanthus amarus), on the other.
View Article and Find Full Text PDFJ Chromatogr B Analyt Technol Biomed Life Sci
December 2012
Department of Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research-CePhaR, Vrije Universiteit Brussel-VUB, Laarbeeklaan 103, B-1090 Brussels, Belgium.
The genera of Mallotus and Phyllanthus contain several species that are commonly used as traditional medicines in oriental countries. Some species show interesting pharmaceutical activities, such as an antioxidant activity. To produce clinically useful medicines or food supplements (nutraceuticals) from these herbs, the species should be identified and a thorough quality control should be implemented.
View Article and Find Full Text PDFAIDS Res Hum Retroviruses
November 1992
Research Institute for Molecular Genetics, Tsumura & Co., Ibaraki-Ken, Japan.
An aqueous extract of Phyllanthus niruri (Euphorbiaceae) inhibited human immunodeficiency virus type-1 reverse transcriptase (HIV-1-RT). The inhibitor against HIV-1-RT in this plant was purified by combination of three column chromatographies, Sephadex LH-20, cellulose, and reverse-phase high-performance liquid chromatography. The inhibitor was then identified by nuclear magnetic resonance (NMR) spectra as repandusinic acid A monosodium salt (RA) which was originally isolated from Mallotus repandus.
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