Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe.

Chem Commun (Camb)

State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, P. R. China.

Published: August 2012

AI Article Synopsis

  • A single-stranded oligonucleotide can cause the aggregation of a perylene probe, resulting in reduced fluorescence from the probe's monomers.
  • When the oligonucleotide has a 5'-phosphate group added by a specific enzyme (polynucleotide kinase), it becomes a target for degradation by lambda exonuclease.
  • This degradation releases the probe monomers and leads to an increase in fluorescence, indicating a "turn-on" signal.

Article Abstract

A single stranded oligonucleotide could induce aggregation of a perylene probe, the probe's monomer fluorescence was efficiently quenched. However, when the oligonucleotide was 5'-phosphorylated by polynucleotide kinase, it could be very efficiently degraded by lambda exonuclease, probe monomers were released, and a turn on fluorescence signal was detected.

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Source
http://dx.doi.org/10.1039/c2cc32644bDOI Listing

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