Runx3 is a transcription factor that belongs to the Runx family. We studied the localization of Runx3 mRNA in the mouse uterus, and its function in the mouse endometrium using Runx3 knockout (Runx3(-/-)) mice. Runx3 mRNA was detected in the endometrial luminal epithelial cells, glandular epithelial cells and stromal cells below the epithelial cell layer on the luminal side. The uteri of Runx3(-/-) mice were smaller than those of wt mice. The endometrial layer and uterine glands of Runx3(-/-) mice were less developed than those of wild-type mice, and the endometrial stromal layer was thinner. Transforming growth factor β1 and β3 (TGFβ1 and β3) mRNA levels in endometrial stromal cells of Runx3(-/-) mice were low compared with those of wild-type mice. Estradiol-17β (E2) increased Tgfb2 mRNA levels in endometrial stromal cells of Runx3(-/-) mice, but not in those of wild-type mice. E2 increased epidermal growth factor (EGF) mRNA levels in endometrial stromal cells of wild-type mice, but did not increase those of Runx3(-/-) mice. The diminished Tgfb1 and Tgfb3 mRNA expressions may lead to the reduced proliferation of endometrial stromal cells. Alterations of E2-associated expressions of Tgfb2 and Egf mRNA in endometrial stromal cells of Runx3(-/-) mice may be associated with suppression of E2-dependent endometrial epithelial cell proliferation in Runx3(-/-) mice. Thus, Runx3 is likely to be a regulatory factor responsible for endometrial growth.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1262/jrd.2012-066 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
An AMBRA1, ULK1 and PP2A regulatory network regulates cytotoxic T cell differentiation via TFEB activation.
Sci Rep
December 2024
Department of Life Sciences, University of Siena, Siena, Italy.
The scaffold protein AMBRA1, which participates in the autophagy pathway, also promotes CD4 T cell differentiation to Tregs independent of autophagy through its interactor PP2A. Here we have investigated the role of AMBRA1 in CD8 T cell differentiation to cytotoxic T cells (CTL). AMBRA1 depletion in CD8 T cells was associated with impaired expression of the transcription factors RUNX3 and T-BET that drive CTL differentiation and resulted in impaired acquisition of cytotoxic potential.
View Article and Find Full Text PDFRunx3, Brn3a and Isl1 interplay orchestrates the transcriptional program in the early stages of proprioceptive neuron development.
PLoS Genet
December 2024
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel.
Article Synopsis
- The study investigates the role of transcription factors Runx3, Brn3a, and Isl1 in the development of sensory proprioceptive neurons, highlighting the serious impact of Runx3 deficiency on neuron survival and motor function.
- The researchers used RNA sequencing and genomic techniques to analyze the interactions and binding sites of these transcription factors in TrkC neurons.
- Their findings reveal that Runx3 primarily interacts with Brn3a to regulate target gene expression through enhancers, while it also plays a distinct role in suppressing immune-related genes.
A polycomb group protein EED epigenetically regulates responses in lipopolysaccharide tolerized macrophages.
Epigenetics Chromatin
November 2024
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Background: To avoid exaggerated inflammation, innate immune cells adapt to become hypo-responsive or "tolerance" in response to successive exposure to stimuli, which is a part of innate immune memory. Polycomb repressive complex 2 (PRC2) mediates the transcriptional repression by catalyzing histone H3 lysine 27 trimethylation (H3K27me3) but little is known about its role in lipopolysaccharide (LPS)-induced tolerance in macrophages.
Result: We examined the unexplored roles of EED, a component of the PRC2, in LPS tolerant macrophages.
Pim1 inactivating induces RUNX3 upregulation that improves/alleviates airway inflammation and mucus hypersecretion in vitro and in vivo.
BMJ Open Respir Res
November 2024
Department of Pediatrics, Yantaishan Hospital, Yantai, Shandong, China
Purpose: Our research aimed to evaluate whether proto-oncogene serine/threonine-protein kinase Pim-1 (Pim1) inactivation could attenuate asthma by promoting runt-related transcription factor 3 (Runx3) expression and explore the underlying molecular mechanism.
Method: Phorbol 12-myristate 13-acetate (PMA, 50 nM) was used to induce inflammation in BEAS-2B human airway epithelial cells. ELISA and immunofluorescence double staining confirmed inflammation modelling and differential expression of Pim1 and Runx3.
Single cell RNA-sequencing delineates CD8 tissue resident memory T cells maintaining rejection in liver transplantation.
Theranostics
September 2024
Organ Transplantation Center, Affiliated Hospital of Qingdao University, Qingdao, China.
Article Synopsis
- Understanding the immune mechanisms in liver transplantation is complicated, especially the role of tissue-resident memory T cells (TRMs).
- The study uses a multi-omics approach, analyzing samples from 17 humans and 16 mice, finding significant insights into T cell behavior.
- There’s a noticeable rise in CD8 TRMs during graft rejection, marked by various activation markers, suggesting they could serve as biomarkers for rejection and help develop better treatment methods for transplant success.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!