AI Article Synopsis
- The study aimed to use synchrotron radiation and microangiographic techniques to identify deep-level microvascular structures in rat hind limbs.
- It involved innovative imaging methods, including in vivo and ex vivo microangiography, using specific contrast media to visualize blood vessels in high detail.
- The findings revealed that small blood vessels down to 9 µm could be effectively visualized, highlighting the potential of these techniques for analyzing microvasculature and angiogenesis in lower limbs.
Article Abstract
Objective: To detect deep-level microvascular structure in rat hind limb by synchrotron radiation and microangiographic technique.
Methods: Microangiography in vivo and ex vivo was performed by synchrotron radiation based absorption imaging and phase contrast imaging, with omnipaque and barium sulfate solution as contrast media, respectively, and synchrotron radiation-based micro-computed tomography (SRmCT) was also performed to reveal three-dimensional morphology of the blood vessel in rat hind limb.
Results: Using microangiographic technique in vivo and in vitro (with barium sulfate), blood vessels in the rat limb muscle could be visualized with high resolution, and the fourth branches of iliac artery in rat hind limb could be detected with the minimum visualized blood vessels about 40 µm and 9 µm in diameter, respectively. In addition, the vascular network could be defined and analyzed at the micrometer scale from the 3D renderings of limb vessel as shown by SRmCT.
Conclusion: Synchrotron radiation-based microangiography and SRmCT thus provided a practical and effective means to observe the microvasculature of rat hindlimb, which might be useful in assessment of angiogenesis in lower limbs.
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