The first example of strip logic gates ("OR" and "AND" functions) for proteins and small molecules has been constructed on the basis of target-induced self-assembly of split aptamer fragments. Using thrombin and ATP as inputs, the corresponding split/integrated aptamers as molecular recognition elements, and gold nanoparticles as a tracer, the output signals can be directly visualized by observing the red bands on the test zones of the strips. The assay is simple, easy to perform, and cost-effective, allowing portable analysis at ambient temperature. The strip logic system is resistant to nonspecific interfering agents and can operate effectively even in human serum samples. Such logic strips hold great promise for application in intelligent point-of-care and in-field diagnostics.
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Article Synopsis
- A novel disulfide linked probe called HTP was developed for the quick detection of copper (Cu) ions, using a reaction between specific chemical compounds and characterized through various spectroscopy techniques and X-ray diffraction.
- The probe exhibits aggregation induced emission (AIE) properties, showing a color change from weak green to intense yellow when binding with Cu ions, making it sensitive to very low concentrations (0.97 nM).
- HTP's reversible interaction with Cu ions can be reset using cysteine, which allows for practical applications in molecular logic gates and detection methods like paper strips and water testing.
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