AI Article Synopsis
- The study investigates the development of Spasmolytic polypeptide-expressing metaplasia (SPEM) in mice and humans, focusing on parietal cell loss and its implications as a preneoplastic lesion.
- Differences in RNA transcript patterns among SPEM from various mouse models were analyzed using gene microarrays and validated with PCR, highlighting key proteins like clusterin and CFTR.
- Results showed that clusterin is consistently upregulated in all SPEM lineages and links to poor survival in gastric cancer, while CFTR expression is specific to inflamed SPEM, suggesting a progression towards a more intestinal-like metaplastic phenotype in inflamed conditions.
Article Abstract
Objectives: Spasmolytic polypeptide-expressing metaplasia (SPEM) develops as a preneoplastic lesion in the stomachs of mice and humans after parietal cell loss. To identify the commonalities and differences between phenotypic SPEM lineages, SPEM were studied from three different mouse models of parietal cell loss: with chronic inflammation with Helicobacter felis infection; with acute inflammation with L635 treatment; and without inflammation following DMP-777 treatment.
Design: RNA transcripts from laser capture microdissected normal chief cells and SPEM lineages were compared using gene microarray. Alterations in transcripts were validated by quantitative real-time PCR. Clusterin and cystic fibrosis transmembrane conductance regulator (CFTR) were selected for immunohistochemical analysis in all mouse models as well as in human SPEM, intestinal metaplasia and gastric cancer.
Results: Transcript expression patterns demonstrated differences among the phenotypic SPEM models. Clusterin expression was significantly upregulated in all three mouse SPEM models as well as in human SPEM. The highest clusterin expression in human gastric cancers correlated with poor survival. Conversely, CFTR expression was upregulated only in SPEM with inflammation in mice. In humans, intestinal metaplasia, but not SPEM, expressed CFTR.
Conclusions: While markers such as clusterin are expressed in all phenotypic SPEM lineages, distinct patterns of upregulated genes including CFTR are present in murine metaplasia associated with inflammation, indicative of progression of metaplasia towards a more intestinalised metaplastic phenotype.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3762676 | PMC |
| http://dx.doi.org/10.1136/gutjnl-2012-302401 | DOI Listing |
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