Vascularization is crucial for implantation of engineered tissues in reconstructive surgery. Polypeptides encapsulated in microspheres can be efficiently transported to their site of action and released in a sustained dosage. We evaluated the effect of delivering vascular endothelial growth factor (VEGF)-encapsulated microspheres in a lipoaspirate scaffold on vascularization and tissue survival. The VEGF-loaded (n=6) and empty (n=6) poly(lactic-co-glycolic acid) microspheres in human lipoaspirate and the human lipoaspirate alone (n=6) were injected subcutaneously into the flanks of athymic nude mice. Three mice from each group were killed, and grafts were explanted at weeks 3 and 6. Increases in mass and volume of VEGF samples, as well as decreases in empty and lipoaspirate-only samples, were observed at 3 and 6 weeks, reaching statistical significance at 6 weeks. Hematoxylin and eosin and CD31+ imaging demonstrated significantly greater vascularization in VEGF samples than in both the empty and lipoaspirate-only groups at both 3 and 6 weeks.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1097/SAP.0b013e3182573827 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Therapeutic Potential of Stromal Vascular Fraction in Enhancing Wound Healing: A Preclinical Study.
Aesthetic Plast Surg
December 2024
Department of Medical Biochemistry, Faculty of Medicine, Istinye University, Istanbul, Turkey.
Background: Adipose tissue provides an abundant source of stromal vascular fraction (SVF) cells for immediate administration. It can also give rise to many multipotent adipose-derived stromal cells. SVF is the population of cells obtained from mechanical or enzymatic digestion of lipoaspirate with no necessity for cell culture or expansion.
View Article and Find Full Text PDF[Experimental substantiation of medical device design for mechanical processing of adipose tissue].
Khirurgiia (Mosk)
December 2024
Petrovsky National Research Center of Surgery, Moscow, Russia.
Objective: To substantiate the medical device design for mechanical processing of adipose tissue.
Material And Methods: Enzymatic method using collagenase and mechanical method for obtaining the stromal vascular fraction (SVF) are compared. The authors emphasized the disadvantages of enzymatic method, such as high cost and duration of process.
Complications of Stem Cell-Based Injections for Knee Osteoarthritis: A Systematic Review.
HSS J
August 2024
Department of Orthopaedic Surgery, University of California, Irvine, Orange, CA, USA.
Article Synopsis
- * A systematic review was conducted following PRISMA guidelines, analyzing studies from Jan 2000 to June 2021 that reported on the adverse events of stem cell therapies for knee OA, focusing on various types of stem cells used in human patients.
- * Out of 1924 patients studied, a 12.3% rate of temporary issues like swelling and pain at the injection site was noted, with umbilical cord-derived and cultured adipose-derived stem cells showing higher rates of complications than other
Minimizing Blood Loss Using Tranexamic Acid in Patients Undergoing Liposuction: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.
Aesthetic Plast Surg
November 2024
King Abdullah International Medical Research Center (KAIMRC), Riyadh, Saudi Arabia.
Background: Liposuction has become the most sought-after aesthetic surgery across the globe, increasing the demand for more developments in patient safety protocols. Evidence of the efficacy of tranexamic acid (TXA) in reducing intraoperative blood loss with minimal complications have led to its widespread applications across various surgical operations. However, the role and effectiveness of TXA in liposuction is not fully understood.
View Article and Find Full Text PDFSubcutaneous delivery of mesenchymal stromal cells induces immunoregulatory effects in the lymph node prior to their apoptosis.
Stem Cell Res Ther
November 2024
Department of Anatomy and Developmental Biology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, 3800, Australia.
Background: Mesenchymal stromal cell (MSC) therapy commonly involves systemic infusion of MSCs, which undergo apoptosis in the lung and induce immunoregulatory macrophages that reduce disease. The relevance of this mode of action, however, is yet to be determined for MSCs administered via other routes. Here, we administered MSCs via subcutaneous (SC) injection into inflamed tissue and investigated the immunomodulatory effects on the local lymph node (LN), which is a major site for the initiation and regulation of immune responses.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!