The tail module subunits of Mediator complex are targets of activators both in yeast and metazoans. Here we discuss recent evidence from studies in yeast for tail module specificity for SAGA-dependent, TATA-containing genes including highly regulated stress response genes, and for independent recruitment and function of the tail module.
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http://dx.doi.org/10.4161/trns.19840 | DOI Listing |
Nat Commun
January 2025
School of Food and Biological Engineering, Engineering Research Center of Bio-process, Ministry of Education, Key Laboratory of Animal Source of Anhui Province, Hefei University of Technology, Hefei, 230009, China.
Dissection of the physiological interactomes of histone post-translational modifications (hPTMs) is crucial for understanding epigenetic regulatory pathways. Peptide- or protein-based histone photoaffinity tools expanded the ability to probe the epigenetic interactome, but in situ profiling in native cells remains challenging. Here, we develop a nucleus-targeting histone-tail-based photoaffinity probe capable of profiling the hPTM-mediated interactomes in native cells, by integrating cell-permeable and nuclear localization peptide modules into an hPTM peptide equipped with a photoreactive moiety.
View Article and Find Full Text PDFJ Vis Exp
December 2024
Department of Epigenetics and Molecular Carcinogenesis, University of Texas MD Anderson Cancer Center; Department of Gynecologic Oncology and Reproductive Medicine, University of Texas MD Anderson Cancer Center;
The CUT&RUN technique facilitates detection of protein-DNA interactions across the genome. Typical applications of CUT&RUN include profiling changes in histone tail modifications or mapping transcription factor chromatin occupancy. Widespread adoption of CUT&RUN is driven, in part, by technical advantages over conventional ChIP-seq that include lower cell input requirements, lower sequencing depth requirements, and increased sensitivity with reduced background signal due to a lack of cross-linking agents that otherwise mask antibody epitopes.
View Article and Find Full Text PDFBiomimetics (Basel)
December 2024
School of Mechatronical Engineering, Beijing Institute of Technology, Beijing 100081, China.
Birds use their claws to perch on branches, which helps them to recover energy and observe their surroundings; however, most biomimetic flapping-wing aircraft can only fly, not perch. This study was conducted on the basis of bionic principles to replicate birds' claw and wing movements in order to design a highly biomimetic flapping-wing aircraft capable of perching. First, a posture conversion module with a multi-motor hemispherical gear structure allows the aircraft to flap, twist, swing, and transition between its folded and unfolded states.
View Article and Find Full Text PDFMol Cell Biol
December 2024
Department of Biology, University of Iowa, Iowa City, Iowa, USA.
Med15 is a general transcriptional regulator and tail module subunit within the RNA Pol II mediator complex. The Med15 protein has a well-structured N-terminal KIX domain, three activator binding domains (ABDs) and several naturally variable polyglutamine (poly-Q) tracts (Q1, Q2, Q3) embedded in an intrinsically disordered central region, and a C-terminal mediator association domain (MAD). We investigated how the presence of ABDs and changes in length and composition of poly-Q tracts influences Med15 activity using phenotypic, gene expression, transcription factor interaction and phase separation assays of truncation, deletion, and synthetic alleles.
View Article and Find Full Text PDFSensors (Basel)
November 2024
College of Engineering Science and Technology, Shanghai Ocean University, Shanghai 201306, China.
The removal of back armor marks the first stage in the comprehensive processing of river crabs. However, the current low level of mechanization undermines the effectiveness of this process. By integrating robotic systems with image recognition technology, the efficient removal of dorsal armor from river crabs is anticipated.
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