AI Article Synopsis
- Matrix effects, including ion suppression and enhancement, pose challenges in high-performance liquid chromatography-mass spectrometry (LC-MS) analyses of biological samples like urine, particularly in doping control.
- This study analyzes 100 urine samples with 41 analytes, offering a new marker to indicate when results may be unreliable due to ion suppression.
- The research also introduces a selective sample preparation method and uses a deuterated internal standard to reduce ion suppression effects and improve the accuracy of glucocorticoid concentration estimations in urine.
Article Abstract
Matrix effects (ion suppression/enhancement) are a well-observed phenomenon in analyses of biological matrices by high-performance liquid chromatography-mass spectrometry (LC-MS). However, few simple solutions for detecting and minimizing these adverse effects have been described so far in multianalyte analysis, especially in the field of doping control. This study describes an exhaustive characterization of matrix effects in one hundred urine samples fortified with 41 analytes (glucocorticoids and diuretics). It introduces a novel marker to identify samples in which the reliability of the results is compromised because of acute ion suppression. This new strategy strengthens the rigor of the analysis for screening purposes. Once the matrix effect is identified, a selective sample preparation is introduced to minimize the adverse ion suppression effect. That selective extraction together with the use of a deuterated internal standard permits enhancing the ruggedness of the estimation of glucocorticoid concentration in urine.
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| http://dx.doi.org/10.1016/j.jchromb.2012.06.007 | DOI Listing |
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