Objectives: Carbon dioxide (CO(2)) insufflation was used by some devices for endoscopic vein harvest to create a subcutaneous tunnel and facilitate the vein harvest. In the literature, some cases of CO(2) micro- and macro-embolisms or hypercarbia during this procedure are described. The purpose of this study was to evaluate if the use of an open CO(2) system rather than a sealed system might be associated with different CO(2) absorption during the procedure.
Methods: Patients were randomized into two groups: those patients in the first group were submitted to endoscopic vein harvest using a sealed CO(2) insufflation; in the second group, the harvest was undertaken with an open CO(2) insufflation. Partial pressure of CO(2) in the arterial blood (PaCO(2)) and end-tidal CO(2) (ETCO(2)) was recorded following anaesthesia induction and before the endoscopic procedure (T0), every 10 min during the endoscopic step (T1) and end after 10 min from the CO(2) insufflation termination (T2).
Results: A total of 60 patients were enrolled. PaCO(2) increased significantly between T0, T1 and T2 in both groups (P = 0.0001) during the endoscopic harvest, but the PaCO(2) level was significantly higher in the group that used the sealed system (44.5 ± 7.9 vs 39.7 ± 7.9 mmHg) at the end of the procedure (P = 0.01). No significant differences between end-tidal CO(2) measured at the same intervals between groups were detected.
Conclusions: There was a constant increase in the blood gas concentration compared with the basal pre-procedure values. Sealed systems were associated with a significantly higher CO(2) concentration at the end of the procedure compared with the open ones.
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http://dx.doi.org/10.1093/icvts/ivs255 | DOI Listing |
Zhongguo Shi Yan Xue Ye Xue Za Zhi
December 2024
Blood Disease Institute, Xuzhou Medical University,Xuzhou 221000, Jiangsu Province, China.
Objective: To harvest the primary Philadelphia chromosome-positive (Ph) cells of B-acute lymphoblastic leukemia (B-ALL) and to establish the B-ALL mouse model.
Methods: The plasmid carrying fusion gene was transferred into the bone marrow(BM) cells of C57BL/6J mice by retrovirus. Syngeneic mice irradiated with 9 Gy of Co γ-ray were injected with the transfected BM cells as the first generation (G1), and then the primary cells from the spleen and BM of the diseased mice were obtained and frozen.
J Vasc Surg
December 2024
Division of Vascular Surgery, Department of Surgery, Henry Ford Hospital, Detroit, USA. Electronic address:
Objective: The impact of great saphenous vein harvest technique on infrainguinal bypass outcomes remains a matter of debate, with no robust evidence favoring a specific technique over the other. This study aims to compare the outcomes of open vein harvest with endoscopic vein harvest in patients undergoing infrainguinal bypass surgery.
Methods: Patients who underwent an infrainguinal bypass from a femoral origin using a single-segment great saphenous vein between 2011 and 2023 were identified in the Vascular Quality Initiative infrainguinal bypass module.
Mymensingh Med J
January 2025
Dr Hitha Antony, Post Graduation 3rd Year Student, Department of Physiology, All India Institute Of Medical Science (AIIMS), Bhopal, India; E-mail:
To assess and compare the sensory component of the saphenous nerve in Coronary artery disease (CAD) patients undergoing Coronary artery bypass graft (CABG) with great saphenous vein graft pre and post-CABG. An observational longitudinal study evaluating the effect of surgery on the saphenous nerve involved 32 CAD patients undergoing CABG. This study was carried out jointly by the Department of Physiology and the Department of Cardiothoracic Vascular Surgery (CTVS) at All India Institute of Medical Sciences, Bhopal, India from November 2022 to January 2024.
View Article and Find Full Text PDFSurg Radiol Anat
December 2024
Faculty of Medicine, Dokuz Eylul University, Izmir, Turkey.
Background: This study investigates the anatomy of the superficial palmar branch of the radial artery (SUPBRA) for palmar skin reconstruction. It aims to detail SUPBRA's anatomical features for hand/finger reconstruction and pinpoint reference points for efficient harvesting.
Materials And Methods: Nineteen male hand specimens (aged 18-70 years), fixed in 10% formalin, were dissected to study the anatomy of SUPBRA island flap.
Front Biosci (Landmark Ed)
November 2024
Department of Breast Surgery, The First People's Hospital of Foshan, 528100 Foshan, Guangdong, China.
Objective: The current study aimed to develop an experimental approach for the direct co-culture of three-dimensional breast cancer cells using single-cell RNA sequencing (scRNA-seq).
Methods: The following four cell culture groups were established in the Matrigel matrix: the untreated Michigan Cancer Foundation (MCF)-7 cell culture group, the MCF-7 cell culture plus cisplatin group, the untreated co-culture group, and the cell co-culture plus cisplatin group. For cell co-culture, MCF-7 cells, human mammary fibroblasts, and human umbilical vein endothelial cells were mixed at a ratio of 1:1:1.
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