Inhibitory effect of IL-1β on galactose intestinal absorption in rabbits.

Cell Physiol Biochem

Unidad de Fisiología, Departamento de Farmacología y Fisiología, Facultad de Veterinaria, Universidad de Zaragoza, Zaragoza, Spain.

Published: October 2012

Background/aims: Recent studies from our laboratory have shown that nitric oxide is involved in the IL-1β-induced inhibition of D-fructose intestinal transport in rabbits. The aim of this work was to further the studies of IL-1β effect on D-galactose absorption in a septic state induced by intravenous administration of this cytokine.

Methods: Galactose intestinal absorption was assessed employing three techniques: sugar uptake in jejunum everted rings, transepithelial flux in Ussing-type chambers and uptake assays in brush border membrane vesicles. The level of the Na(+)/D-glucose cotransporter (SGLT1) expression was analyzed by Western blot.

Results: In sepsis condition the body temperature was increased and studies on cellular intestinal integrity have not shown modifications in the brush border membrane. However, D-galactose absorption across mucosa of jejunum was diminished in IL-1β treated rabbits. The levels of SGLT-1 were no significantly different in both animal groups (control and IL-1β treated), indicating that the cytokine could induce a reduction in the SGLT-1 functionality. The inhibition was significantly reversed by the activation of several PKC, PKA, MAPKs and nuclear factor (NF)-ĸB inhibitors administered 15 min before the IL-1β.

Conclusion: The inhibitory effect of IL-1β on D-galactose absorption across mucosal side of enterocyte could be mediated by the activation of several kinases and nuclear factor (NF)-ĸB.

Download full-text PDF

Source
http://dx.doi.org/10.1159/000339056DOI Listing

Publication Analysis

Top Keywords

d-galactose absorption
12
inhibitory il-1β
8
galactose intestinal
8
intestinal absorption
8
il-1β d-galactose
8
brush border
8
border membrane
8
il-1β treated
8
nuclear factor
8
factor nf-ĸb
8

Similar Publications

Currently, the utilization value of plantain straw is low. To increase its value, plantain straw was utilized in this study to produce xylooligosaccharides (XOS). XOS were obtained from plantain straw through xylanase hydrolysis.

View Article and Find Full Text PDF

Biochemical Characteristics of Urine Metabolomics in Female Giant Pandas at Different Estrous Stages.

Animals (Basel)

December 2024

Chengdu Research Base of Giant Panda Breeding, Chengdu 610081, China.

The composition of urinary metabolites can reflect the physiological state of animals. Investigating the alterations in urine metabolomics during the estrus stage can provide valuable insights for enhancing the efficacy of estrus monitoring. This study aimed to perform an analysis of urinary metabolomics in female giant pandas, specifically examining the variations in specific metabolites across different estrous stages, namely, diestrus, proestrus, estrus, and metestrus.

View Article and Find Full Text PDF

Resveratrol (Res), a Chinese herbal extract, has demonstrated a remarkable and distinct antitumor effect, characterized by prolonged efficacy and minimal adverse reactions. However, the bioavailability of Res in animals is hindered by limited absorption rates. Therefore, it is crucial to enhance the tumor-targeting ability of resveratrol to optimize cancer treatment outcomes by improving its bioavailability.

View Article and Find Full Text PDF
Article Synopsis
  • - Polysaccharides were extracted from leaves (WSPRaL) and stems (WSPRaS) of a plant, yielding about 3% and 3.25%, respectively, with varying compositions of proteins, lipids, and sugars.
  • - Detailed analyses showed that WSPRaL and WSPRaS contain different types of carbohydrates, with SEM and FT-IR used to examine their microstructure and physicochemical properties.
  • - The polysaccharides demonstrated strong antioxidant, antibacterial, and biofilm-inhibitory activities, suggesting their potential as functional ingredients in the food and nutraceutical industries.
View Article and Find Full Text PDF

Response mechanism of Saccharomyces cerevisiae under benzoic acid stress in ethanol fermentation.

Sci Rep

November 2024

College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou, 545006, People's Republic of China.

Sugarcane molasses is an ideal economical raw material for ethanol production because of its wide availability, low cost and nutrient content. However, benzoic acid compounds with toxic effects on yeast cells are commonly found in sugarcane molasses. At present, the molecular mechanism of the toxic effects of benzoic acid on Saccharomyces cerevisiae has not been elucidated.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!