AI Article Synopsis
- D-alanyl lipoteichoic acids in Gram-positive bacteria are essential for autolysis, cation balance, and biofilm formation.
- The D-alanylation process involves the D-alanyl carrier protein DltC, which transfers D-Ala to membrane-associated LTAs.
- The study involved purifying and crystallizing DltC from Staphylococcus epidermidis, leading to a crystal resolution of 1.83 Å and providing insights into its functional role in LTA alanylation.
Article Abstract
The D-alanyl lipoteichoic acids (D-alanyl LTAs) present in the cell walls of Gram-positive bacteria play crucial roles in autolysis, cation homeostasis and biofilm formation. The alanylation of LTAs requires the D-alanyl carrier protein DltC to transfer D-Ala onto a membrane-associated LTA. Here, DltC from Staphylococcus epidermidis (SeDltC) was purified and crystallized using the sitting-drop vapour-diffusion method. The crystals diffracted to a resolution of 1.83 Å and belonged to space group P2, with unit-cell parameters a = 66.26, b = 53.28, c = 88.05 Å, β = 98.22°. The results give a preliminary crystallographic analysis of SeDltC and shed light on the functional role of DltC in the alanylation of LTAs.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3388928 | PMC |
| http://dx.doi.org/10.1107/S1744309112021720 | DOI Listing |
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