Development and application of an in-house reverse hybridization method for Chlamydia trachomatis genotyping.

J Appl Microbiol

Department of Microbiology and research laboratory Microorganismes et Pathologie Humaine, Habib Bourguiba university hospital, Medical school of Sfax, University of Sfax, Sfax, Tunisia.

Published: October 2012

Aim: To develop and evaluate an in-house reverse hybridization technique for Chlamydia trachomatis genotype identification.

Methods And Results: The evaluation of the developed and optimized reverse hybridization method on reference strains showed the specific detection of all genotypes. This technique showed its ability to type one inclusion-forming unit of C. trachomatis genotype E and equivalent sensitivity to the Cobas TaqMan assay. It was also able to detect mixed infections in vitro. Application of the reverse hybridization method on 38 isolated C. trachomatis strains and their respective swabs allowed the detection of six urogenital genotypes D, E, F, G, H and K and one trachoma genotype B. Genotype E was the most prevalent, detected in 73% of the swab samples. Mixed infections were detected in 26% of swab cases.

Conclusion: The reverse hybridization technique is simple and does not require specialized instruments. It is powerful in the diagnosis of mixed infections and is suitable for use in epidemiological studies.

Significance And Impact Of The Study: This technique allowed rapid C. trachomatis genotype identification.

Download full-text PDF

Source
http://dx.doi.org/10.1111/j.1365-2672.2012.05375.xDOI Listing

Publication Analysis

Top Keywords

reverse hybridization
20
hybridization method
12
mixed infections
12
in-house reverse
8
chlamydia trachomatis
8
hybridization technique
8
c trachomatis genotype
8
reverse
5
hybridization
5
genotype
5

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!