The key target of green chemistry is to make compounds and materials available to mankind, while causing no harm to the environment. In the 21st century analytical scientists are more concerned about green analytical method development. The number of literatures on green chemistry has undergone a dramatic increase in the new millennium. Green bioanalytical techniques aim to minimize or eliminate the hazardous waste associated with bioanalytical methods. An efficient and sincere approach towards bioanalytical method development has an enormous contribution towards green analysis. The selection of organic constituents of the mobile phase, choice of sample extraction process, adoption of an appropriate separation procedure and a few others, control the green chemistry approach of the bioanalytical method. In routine practice, UHPLC-MS can be the most suitable approach, while supercritical fluid chromatography is one of the best available techniques for green bioanalytical methods. Nevertheless, there always remains great scope of further research on green bioanalytical methods.
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