AI Article Synopsis
- The study investigates the complex chromosomal changes in head and neck squamous cell carcinoma (HNSCC), particularly focusing on the role of DNA methylation in centromeric instability.
- Researchers analyzed the methylation status of various DNA repeat sequences and their relationship to chromosomal abnormalities in HNSCC cell lines and tumours.
- Results indicated a significant link between hypomethylation of LINE-1 repeats and centromeric instability, suggesting that genomic DNA hypomethylation is specifically associated with certain repeat sequences in these tumours.
Article Abstract
Background: Head and neck squamous cell carcinoma (HNSCC) is a tumour type that generally carries very complex chromosomal aberrations. An interesting feature is the elevated occurrence (58 %) of whole arm translocations and isochromosomes, resulting from breakage and illegitimate recombination in centromeric or pericentromeric regions. We hypothesized that alterations in DNA methylation may play a role in the breakage of centromeric repeat sequences in these tumours.
Methods: We studied the DNA methylation status of global repeats (LINE-1), subtelomeric repeats (D4Z4) and centromeric repeats (SAT-α) in relation to centromeric instability in a series of HNSCC cancer cell lines and primary tumours. We analysed the methylation status by pyrosequencing and the chromosomal aberrations by microarray CGH.
Results: We found a significant association between centromeric instability and hypomethylation of LINE-1, but not D4Z4 and SAT-α.
Conclusion: These data suggest that centromeric instability is associated with genomic DNA hypomethylation only when occurring at specific DNA repeat sequences.
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| http://dx.doi.org/10.1007/s13402-012-0085-5 | DOI Listing |
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