The influence of both acidic and basic hydrolysis on the yield, total phenolic content and antioxidative capacity of methanolic extract of germinated brown rice (GBR) was studied. Total phenolic content (TPC), total flavonoid content (TFC), 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation scavenging, and ferric reducing antioxidant power (FRAP) tests were used for the measurement of antioxidant ability. There was a significant difference p < 0.05) in the TPC and DPPH radical scavenging assay results when comparing neutral with acidic and basic catalysed hydrolysis. The yield of the crude extract was slightly higher in acidic hydrolysis than in basic hydrolysis p > 0.05). The TPC and TFC were highest in acidic hydrolysis. A significant correlation was observed between ABTS radical cation scavenging and FRAP. The antioxidant activity measured using DPPH radical scavenging assay showed high activity in acidic hydrolysis, while the ABTS radical cationscavenging activity and FRAP showed the highest values in basic hydrolysis. The samples were further evaluated using HPLC to determine the individual phenolic concentrations in different hydrolytic media contributing to the antioxidant effects. This study revealed that acidic and basic hydrolysis can improve the yield, phenolic content, and antioxidant activity of germinated brown rice.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6268343 | PMC |
| http://dx.doi.org/10.3390/molecules17067584 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Anticarcinogenic cationic peptides derived from tryptic hydrolysis of β-lactoglobulin.
Front Mol Biosci
January 2025
Department of Biochemistry, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
Introduction: This study investigated the tryptic hydrolysis of β-lactoglobulin (BLG) for 30, 60, 90, and 120 min at 1/200 E/S (enzyme/substrate ratio, w/w) to prepare potentially anticarcinogenic peptides.
Methods: The properties of hydrolysates were characterized, including degree of hydrolysis, free amino acids, SDS-PAGE, FTIR, and antioxidant activity employing DPPH-assay, β-carotene/linoleic acid, and FRAP assay.
Results: BLG tryptic hydrolysate produced after 60 min hydrolysis recorded the highest antioxidant activity, and LCMS analysis revealed 162 peptides of molecular masses ranging from 800 to 5671Da, most of them are of hydrophobic nature.
GPIHBP1 increase accounts for rheumatic arthritis-related hypotriglyceridemia by facilitating lipids uptake of white adipose tissues.
Arthritis Res Ther
January 2025
Xin'an Medicine Research Center, the First Affiliated Hospital of Wannan Medical College (Yijishan Hospital), No 2, West Zheshan Road, Wuhu, Anhui, 241000, China.
Background: Metabolism alteration is a common complication of rheumatic arthritis (RA). This work investigated the reason behind RA-caused triglyceride (TG) changes.
Methods: Fresh RA patients' whole blood was transfused into NOD-SCID mice.
Regulation of Zinc Hydroxide Sulfate Growth Environment for Stable Zinc Anode/Electrolyte Interfaces.
Langmuir
January 2025
College of Materials Science and Engineering, Sichuan University, Chengdu 610064, China.
Metallic Zn is a promising anode for high-safety, low-cost, and large-scale energy storage systems. However, it is strongly hindered by unstable electrode/electrolyte interface issues, including zinc dendrite, corrosion, passivation, and hydrogen evolution reactions. In this work, an in situ interface protection strategy is established by turning the corrosion/passivation byproducts (zinc hydroxide sulfates, ZHSs) into a stable hybrid protection layer.
View Article and Find Full Text PDFBranching and molecular weight in levan: A detailed analysis of structural variability and enzymatic hydrolysis susceptibility.
Carbohydr Polym
March 2025
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001 Chamilpa, 62210 Cuernavaca, Morelos, Mexico. Electronic address:
Levan, a β(2 → 6) linked D-fructofuranosyl polymer, is gaining significant attention in basic and applied research. It has been demonstrated that most properties are related to levan molecular weight but also its β(2 → 1) branching degree. In this paper the relationship between levan branching degree, particle size, and molecular weight is reviewed, exploring also how these structural parameters influence levan susceptibility to exo- and endolevanase hydrolysis for levans produced by three recombinants bacterial levansucrases.
View Article and Find Full Text PDFImpact of humidity on the adsorption and decomposition of mustard gas simulant on heteroatom-modified nanoporous carbon textiles.
J Hazard Mater
January 2025
Department of Chemistry and Biochemistry, The City College of New York, 160 Convent Ave., New York, NY 10031, United States. Electronic address:
Activated carbon textile (C-Text) was chemically modified to incorporate oxygen- (C-Text-O), nitrogen- (C-Text-ON), and/or sulfur- (C-Text-OS) containing surface functional groups, aiming to enhance their reactive adsorption capacity. The modified textiles were evaluated for their ability to detoxify 2-choloroethyl ethyl sulfide (CEES) in both vapor and liquid phases, under dry and humid conditions. The maximum amount of water adsorbed was directly affected by the surface area (R = 0.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!