AI Article Synopsis
- Calcium-dependent antibiotics (CDA) are a type of cyclic lipopeptide created using nonribosomal peptide synthetase (NRPS) enzymes.
- Researchers modified the active site of a specific domain in the CDA NRPS to allow the incorporation of a synthetic amino acid, 3-methyl glutamine, into the peptide structure.
- This work demonstrates a novel method for engineering A-domains in NRPS to add synthetic "non-natural" amino acids into nonribosomal peptides, expanding the potential for new antibiotic development.
Article Abstract
Calcium-dependent antibiotics (CDA) are cyclic lipopeptides assembled by nonribosomal peptide synthetase (NRPS) enzymes. Active site modification of the 3-methyl glutamate activating adenylation (A) domain of the CDA NRPS enables the incorporation of synthetic 3-methyl glutamine into CDA. This provides the first example of how A-domains can be engineered to introduce synthetic "non-natural" amino acids into nonribosomal peptides.
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| http://dx.doi.org/10.1002/anie.201202043 | DOI Listing |
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