AI Article Synopsis
- The study investigates the effects of 27-hydroxycholesterol (27OHC) on estrogen-dependent proteins in MCF7 cancer cells, which have estrogen receptors.
- Results show that 27OHC functions similarly to estrogen (E2) in regulating proteins like MnSOD and ERβ, suggesting it acts as a selective estrogen receptor modulator (SERM).
- Additionally, prolonged exposure to 27OHC leads to epithelial to mesenchymal transition (EMT) in MCF7 cells, with a decrease in nuclear FOXM1 levels, highlighting potential implications for obese patients with certain types of breast cancer.
Article Abstract
The principal aim of this study was to analyze in estrogen receptor-positive MCF7 cells the response of three estrogen-dependent proteins to 27-hydroxycholesterol (27OHC), a major circulating cholesterol metabolite. Immunofluorescence, immunoblotting and immunogold labelling analyses of MCF7 cells exposed for up to 72 h to 2 nM estradiol (E2) or to 2 µM 27OHC demonstrated similar responses in the expression of MnSOD and ERβ compared to the non-stimulated cells. Thus, the results confirm 27OHC's function as a novel selective estrogen receptor modulator (SERM). The epithelial to mesenchymal transition (EMT), observed in MCF7 cells stimulated for longer than 48 h with 2 µM 27OHC, was accompanied by lower immunoreactive levels of nuclear FOXM1 in comparison to E2-treated cells. The results presented in this study are discussed taking into consideration the relationship of hypercholesterolemia, 27OHC production, ROS synthesis and macrophage infiltration, potentially occurring in obese patients with ERα-positive, infiltrated mammary tumors.
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| http://dx.doi.org/10.3892/or.2012.1859 | DOI Listing |
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