Large-scale development of expressed sequence tag simple sequence repeat (EST-SSR) markers was performed in peanut (Arachis hypogaea L.) to obtain more informative genetic markers. A total of 10,102 potential non-redundant EST sequences, including 3,445 contigs and 6,657 singletons, were generated from cDNA libraries of the gynophore, roots, leaves and seedlings. A total of 3,187 primer pairs were designed on flanking regions of SSRs, some of which allowed one and two base mismatches. Among the 3,187 markers generated, 2,540 (80%) were trinucleotide repeats, 302 (9%) were dinucleotide repeats, and 345 (11%) were tetranucleotide repeats. Pre-polymorphic analyses of 24 Arachis accessions were performed using 10% polyacrylamide gels. A total of 1,571 EST-SSR markers showing clear polymorphisms were selected for further polymorphic analysis with a Fluoro-fragment Analyzer. The 16 Arachis accessions examined included cultivated peanut varieties as well as diploid species with the A or B genome. Altogether 1,281 (81.5%) of the 1,571 markers were polymorphic among the 16 accessions, and 366 (23.3%) were polymorphic among the 12 cultivated varieties. Diversity analysis was performed and the genotypes of all 16 Arachis accessions showed similarity coefficients ranging from 0.37 to 0.97. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-011-9604-8) contains supplementary material, which is available to authorized users.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3362703 | PMC |
| http://dx.doi.org/10.1007/s11032-011-9604-8 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Reduced-Cost Genotyping by Resequencing in Peanut Breeding Programs Using Tecan Allegro Targeted Resequencing V2.
Genes (Basel)
October 2024
Department of Plant and Soil Science, Texas Tech University, Lubbock, TX 79409, USA.
The identification of informative molecular markers is useful for linkage mapping and can benefit genome-wide association studies by providing fine-scale information about sequence variations. However, high-throughput genotyping approaches are not cost-effective for labs that require frequent use, such as breeding programs that need to perform genotyping on large populations with hundreds of individuals. The number of single nucleotide polymorphism markers generated by those approaches can be far more than needed for most breeding programs; instead, breeders focus on the use of at most hundreds of polymorphic molecular markers for analysis.
View Article and Find Full Text PDFResponse to oxalic acid: an important supplement screening against stem rot resistance in groundnut (Arachis hypogaea L.).
BMC Plant Biol
November 2024
International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, 502324, India.
Background: Stem rot, caused by the soil-borne pathogen Sclerotium rolfsii, pose a serious challenge in the groundnut (Arachis hypogaea L) cultivation. Although this disease is widespread globally but had most adverse impact in groundnut growing regions of United States, India, and Australia. The pathogen primarily targets the crown region of the plant, resulting in systemic collapse and potentially leading to yield losses up to 80%.
View Article and Find Full Text PDFIdentification and Characterization of Peanut Seed Coat Secondary Metabolites Inhibiting Growth and Reducing Aflatoxin Contamination.
J Agric Food Chem
October 2024
Department of Plant and Soil Science, Texas Tech University, Lubbock, Texas 79409, United States.
The peanut seed coat acts as a physical and biochemical barrier against infection; however, the nature of the inhibitory chemicals in the peanut seed coat in general is not known. This study identified and characterized peanut seed coat metabolites that inhibit growth and aflatoxin contamination. Selected peanut accessions grown under well-watered and water-deficit conditions were assayed for resistance, and seed coats were metabolically profiled using liquid chromatography mass spectrometry.
View Article and Find Full Text PDFCo-localization of quantitative trait loci for pod and kernel traits and development of molecular marker for kernel weight on chromosome Arahy05 in peanut (Arachis hypogaea L.).
Theor Appl Genet
October 2024
College of Agriculture, Nanjing Agricultural University, Nanjing, 210095, China.
Article Synopsis
- Researchers identified stable quantitative trait loci (QTL) for important pod and kernel traits in peanuts, located on chromosome Arahy05, which are crucial for yield improvement in breeding programs.
- An INDEL marker developed at position 106,411,957 on Arahy05 has been validated for its effectiveness in marker-assisted selection (MAS) of kernel weight.
- The study also discovered two main haplotypes linked to kernel weight, with specific botanical varieties showing either increased or decreased hundred kernel weight, potentially guiding breeding strategies.
Genome-wide association study reveals the genetic basis of amino acids contents variations in Peanut (Arachis hypogaea L.).
Physiol Plant
October 2024
Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Crop Genetic Improvement, South China Peanut Sub-Centre of National Centre of Oilseed Crops Improvement, Guangzhou, China.
Peanut is a significant source of protein for human consumption. One of the primary objectives in peanut breeding is the development of new cultivars with enhanced nutritional values. To further this goal, a genome-wide association study (GWAS) was conducted to analyze seed amino acids contents in 390 diverse peanut accessions collected worldwide, mainly from China, India, and the United States, in 2017 and 2018.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!