AI Article Synopsis
- The study aimed to investigate how cytokine gene expression changes during the cultivation of leukocytes from healthy donors.
- RNA from leukocytes was analyzed at different time points (0, 3, and 24 hours), focusing on 8 cytokine genes using PCR techniques.
- Results showed that after 3 hours, gene expression for pro-inflammatory cytokines peaked, while expression levels of anti-inflammatory cytokines remained stable over 24 hours.
Article Abstract
Aim: Study of expression of cytokine genes in the process of cultivation of healthy donor leukocytes.
Materials And Methods: RNA isolated before, after 3 and 24 hours of cultivation of leukocytes of 15 healthy donors aged 19 - 32 years was the object of the study. Study of the expression of genes of 8 cytokines by the level of their mRNA was performed by using polymerase chain reactionwith reverse transcription.
Results: In healthy donors among with individual differences, general regularities were traced: higher levels of IFNalpha and anti-inflammatory cytokine gene expression, as well as predominantly low expression of pro-inflammatory cytokines.
Conclusion: In most of the cases in the process of cultivation of leukocytes isolated from blood donors the highest level of gene expression of pro-inflammatory cytokines is noted after 3 hours of cultivation, and expression of genes of anti-inflammatory cytokines remains constant for 24 hours of cultivation.
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