Bacteriophages are associated with reduced fecal shedding of Shiga-toxin-producing Escherichia coli O157:H7 (STEC O157:H7) in cattle. Four phages exhibiting activity against 12 of 14 STEC O157:H7 strains, representing 11 common phage types, were isolated. Phages did not lyse non-O157 E. coli, with 11 of the 12 STEC strains exhibiting extreme susceptibility (average multiplicity of infection (MOI) = 0.0003-0.0007). All phages had icosahedral heads with tapered, noncontractile tails, a morphology indicative of T1-like Siphoviridae. Genome size of all phages was ∼44 kb, but EcoRІ or HindIII digestion profiles differed among phages. Based on restriction enzyme digestion profiles, phages AHP24, AHS24, and AHP42 were more related (66.7%-82.4%) to each other than to AKS96, while AHP24 and AHS24, isolated from the same feedlot pen, exhibited the highest identity (88.9%-92.3%). Phages AHP24 and AHS24 exhibited the broadest host range and strongest lytic activity against STEC O157:H7, making them strong candidates for biocontrol of this bacterium in cattle.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1139/w2012-063 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Detection of O157H7 and in Bovine Carcasses in Two Slaughterhouses in Bio-Bío District, Chile.
Foodborne Pathog Dis
July 2024
Facultad de Medicina Veterinaria y Agronomía, Universidad de Las Américas, Concepción, Chile.
Article Synopsis
- * Out of 544 samples analyzed from 272 bovine carcasses, 11 tested positive for O157:H7 (4.0%), and 6 of those had specific pathogenicity genes detected.
- * Additionally, 22 carcasses (8.0%) tested positive for the unspecified pathogen, but none contained the specific genes associated with pathogenicity.
Analysis of drug sensitivity of Escherichia Coli O157H7.
Biomed Microdevices
May 2021
College of information science and engineering, Shanxi Agricultural University, Jinzhong, 030800, People's Republic of China.
Sensitive and rapid tests of Escherichia coli drug sensitivity is very important for health of human and animals. An E. coli immunosensor was built based on electrochemical detection and immune detection technologies, through pretreating screen-printed electrodes, and analyzing the optimal reaction concentration of antigen antibody binding with the AC impedance method.
View Article and Find Full Text PDFThe progress of type II persisters of O157:H7 to a non-culturable state during prolonged exposure to antibiotic stress with revival being aided through acid-shock treatment and provision of methyl pyruvate.
Can J Microbiol
July 2021
Center of Public Health and Zoonosis, Department of Food Science, University of Guelph, Guelph, Ontario, Canada.
Persisters are a form of dormancy in bacteria that provide temporary resistance to antibiotics. The following reports on the formation of O157:H7 E318 type II persisters from a protracted (8 days) challenge with ampicillin. O157:H7 followed a multiphasic die-off pattern with an initial rapid decline (Phase I) of susceptible cells that transitioned to a slower rate representing tolerant cells (Phase II).
View Article and Find Full Text PDFCombination of garlic essential oil, allyl isothiocyanate, and nisin Z as bio-preservatives in fresh sausage.
Meat Sci
September 2018
School of Life Sciences, Pontifícia Universidade Católica do Paraná, Rua Imaculada Conceição-1155, 80215-901 Curitiba, Brazil. Electronic address:
The effects of natural antimicrobial compounds (garlic essential oil [GO], allyl isothiocyanate [AITC], and nisin Z [NI]) on microbiological, physicochemical and sensory characteristics of fresh sausage were assessed. The minimum inhibitory concentrations (MICs) and the minimum bactericidal concentrations (MBCs) towards Escherichia coli O157:H7 and Lactobacillus plantarum were determined in vitro. Sausages inoculated with E.
View Article and Find Full Text PDFGlobal genome response of Escherichia coli O157∶H7 Sakai during dynamic changes in growth kinetics induced by an abrupt temperature downshift.
PLoS One
October 2015
Food Safety Centre, Tasmanian Institute of Agriculture, University of Tasmania, Hobart, Tasmania, Australia.
Escherichia coli O157∶H7 is a mesophilic food-borne pathogen. We investigated the growth kinetics of E. coli O157∶H7 Sakai during an abrupt temperature downshift from 35°C to either 20°C, 17°C, 14°C or 10°C; as well as the molecular mechanisms enabling growth after cold stress upon an abrupt downshift from 35°C to 14°C in an integrated transcriptomic and proteomic analysis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!