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Standard Reference Material (SRM) 3278 Tocopherols in Edible Oils has been issued for use as a quality assurance tool in the measurement of tocopherols. Like other natural-matrix SRMs, this material can be used in method validation or in assignment of tocopherol values to in-house quality control materials. Because most edible oils contain one predominant tocopherol isoform, the SRM is a blend of sunflower, soy, canola, and safflower oils to provide roughly comparable chromatographic peak heights of the two main tocopherols, γ and α, with smaller amounts of δ and β. The four tocopherol isoforms were determined by three independent liquid chromatography methods with absorbance and fluorescence detection. Various chromatographic and detection modes are used for assignment of certified values because biases inherent to one method should not be present in the other, and the existence of bias can therefore be identified.
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