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A Simple Protocol for Visualization of RNA-Protein Complexes by Atomic Force Microscopy.
Curr Protoc
January 2025
Czech Metrology Institute, Brno, Czech Republic.
Atomic force microscopy (AFM) has recently received increasing interest in molecular biology. This technique allows quick and reliable detection of biomolecules. However, studying RNA-protein complexes using AFM poses significant challenges.
View Article and Find Full Text PDFStructural basis for the transport and regulation mechanism of the multidrug resistance-associated protein 2.
Nat Commun
January 2025
Laboratory of Membrane Biology and Biophysics, The Rockefeller University, New York, NY, USA.
Multidrug resistance-associated protein 2 (MRP2) is an ATP-powered exporter important for maintaining liver homeostasis and a potential contributor to chemotherapeutic resistance. Using cryogenic electron microscopy (cryo-EM), we determine the structures of human MRP2 in three conformational states: an autoinhibited state, a substrate-bound pre-translocation state, and an ATP-bound post-translocation state. In the autoinhibited state, the cytosolic regulatory (R) domain plugs into the transmembrane substrate-binding site and extends into the cytosol to form a composite ATP-binding site at the surface of nucleotide-binding domain 2.
View Article and Find Full Text PDFEvaluation of minced beef quality fortified with edible microalgae species during cryogenic storage.
Food Res Int
January 2025
Chemistry of Natural Compounds Department, National Research Centre, 33 El-Behouth St, Dokki-Giza 12622, Egypt. Electronic address:
The aim of this study is to evaluate the effect of some microalgae species adding with different forms on minced beef meat shelf life during cryogenic storage for 13 days. Chlorella vulgaris and Arthrospira platensis are chosen because of their safety and high nutritional value. Microalgae nanoparticles with their different forms have been prepared by using emulsification solvent evaporation method.
View Article and Find Full Text PDFAdvanced Analysis of Clay Microporosity Using High-Resolution N-Ar Adsorption Isotherms Coupled with the Derivative Isotherm Summation Method.
Molecules
December 2024
IMT Atlantique, GEPEA, UMR CNRS 6144, F-44307 Nantes, France.
The textural properties of synthetic and natural clays in the sodium form and exchanged with tetramethylammonium cations (TMA) were characterized using N and Ar physisorption isotherms at cryogenic temperatures. Specific surface areas and micro/mesoporous volumes were determined using the BET and the models. The analysis requires the use of reference isotherms measured at the same temperature on the surface of non-porous materials with an identical chemical composition.
View Article and Find Full Text PDFMethodological Assessment of ExoGAG for Isolation of Cerebrospinal Fluid Extracellular Vesicles as a Source of Biomarkers.
Int J Mol Sci
December 2024
Centro de Investigación en Red-Enfermedades Neurodegenerativas (CIBERNED), 19171 Madrid, Spain.
Extracellular vesicles (EVs) in cerebrospinal fluid (CSF) represent a valuable source of biomarkers for central nervous system (CNS) diseases, offering new pathways for diagnosis and monitoring. However, existing methods for isolating EVs from CSF often prove to be labor-intensive and reliant on specialized equipment, hindering their clinical application. In this study, we present a novel, clinically compatible method for isolating EVs from CSF.
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