AI Article Synopsis
- PACAP is a neuroprotective peptide found in the central nervous system, and its role in spinal cord injury (SCI) was studied using both PACAP knockout and wild-type mice.
- Real-time PCR showed that PACAP mRNA levels increased progressively for 14 days post-SCI, indicating a response to the injury, while PAC1R levels also rose.
- Wild-type mice demonstrated improved motor function recovery over 14 days, whereas PACAP knockout mice had significantly poorer recovery, suggesting that endogenous PACAP plays a crucial role in protecting against spinal cord damage.
Article Abstract
Pituitary adenylate cyclase-activating polypeptide (PACAP) is a neuroprotective peptide expressed in the central nervous system. To date, changes in the expression and effect of endogenous PACAP have not been clarified with respect to spinal cord injury (SCI). The aim of this study was to elucidate the expression pattern and function of endogenous PACAP on the contusion model of SCI using heterozygous PACAP knockout (PACAP(+/-)) and wild-type mice. Real-time polymerase chain reaction methods revealed that the level of PACAP mRNA increased gradually for 14 days after SCI and that PAC1R mRNA levels also increased for 7 days compared with intact control mice. PACAP and PAC1R immunoreactivities colabeled with a neuronal marker in the intact spinal cord. Seven days after SCI, PAC1R immunoreactivity was additionally co-expressed with an astrocyte marker. Wild-type mice gradually recovered motor function after 14 days, but PACAP(+/-) mice showed significantly impaired recovery from 3 days compared with wild-type mice. The injury volume at day 7 in PACAP(+/-) mice, and the number of single-stranded DNA-immunopositive cells as a marker of neuronal cell death at day 3 were significantly higher than values measured in wild-type mice. These data suggest that endogenous PACAP is upregulated by SCI and has a neuroprotective effect on the damaged spinal cord.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s12031-012-9817-2 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cognitive Deficits in Anti-LGI1 Encephalitis Are Linked to Immunotherapy-Resistant White Matter Network Changes.
Neurol Neuroimmunol Neuroinflamm
March 2025
Department of Neurology and Experimental Neurology, Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt- Universität zu Berlin.
Background And Objectives: Cognitive deficits represent a major long-term complication of anti-leucine-rich, glioma-inactivated 1 encephalitis (LGI1-E). Although severely affecting patient outcomes, the structural brain changes underlying these deficits remain poorly understood. In this study, we hypothesized a link between white matter (WM) networks and cognitive outcomes in LGI1-E.
View Article and Find Full Text PDFUser-Centered Design of Neuroprosthetics: Advancements and Limitations.
CNS Neurol Disord Drug Targets
January 2025
Biosciences and Bioengineering PhD Program, American University of Sharjah, UAE.
Neurological conditions resulting from severe spinal cord injuries, brain injuries, and other traumatic incidents often lead to the loss of essential bodily functions, including sensory and motor capabilities. Traditional prosthetic devices, though standard, have limitations in delivering the required dexterity and functionality. The advent of neuroprosthetics marks a paradigm shift, aiming to bridge the gap between prosthetic devices and the human nervous system.
View Article and Find Full Text PDFCase report: Movement-related neuroplasticity in a patient after spinal cord injury in response to task-oriented bimanual training.
Front Hum Neurosci
January 2025
Department of Rehabilitation Sciences, The Hong Kong Polytechnic University, Kowloon, Hong Kong SAR, China.
Background: Bimanual motor training is an effective neurological rehabilitation strategy. However, its use has rarely been investigated in patients with paralysis caused by spinal cord injury (SCI). Therefore, we conducted a case study to investigate the effects of robot-assisted task-oriented bimanual training (RBMT) on upper limb function, activities of daily living, and movement-related sensorimotor activity in a patient with SCI.
View Article and Find Full Text PDFA Rare Case of Bilateral Foot Drop Following Cervical Decompression in Tandem Spinal Stenosis.
Cureus
December 2024
Orthopaedic Surgery, Ng Teng Fong General Hospital, Singapore, SGP.
This case report describes a 70-year-old male presenting with limb weakness, urinary retention and tandem cervical and lumbar spinal stenosis with complicating white cord syndrome, a rare reperfusion injury post decompression surgery. Initially admitted following an unwitnessed fall, the patient's neurological examination indicated that progressive weakness of the limbs and sensory loss etiology is cervical and lumbar spondylosis with severe spinal canal stenosis, confirmed by imaging. Due to rapid deterioration, he underwent C5 corpectomy, cervical decompression and fusion.
View Article and Find Full Text PDFConsumer Products Nerve Injuries Among Patients Products Presenting to United States Emergency Departments Between 2012 and 2021: A Nationwide Cohort.
Open Access Emerg Med
January 2025
Department of Anesthesiology, Warren Alpert School of Medicine, Brown University, Providence, RI, 02903, USA.
Introduction: Nerve injuries and resultant pain are common causes of emergency department (ED) visits in the United States. Injuries often occur either due to activity (ie sports related injury) or due to consumer products such as stairs or bedframes. We investigated the incidence of consumer product-related nerve injuries (CPNIs) in patients who presented to the ED in the United States.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!