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Cold response of dedifferentiated barley cells at the gene expression, hormone composition, and freezing tolerance levels: studies on callus cultures. | LitMetric

AI Article Synopsis

  • - The study investigates how dark-grown, embryogenic barley callus cells react to cold without relying on light or chloroplasts, measuring the expression of key cold response genes (HvCBF9, HvCBF14, HvCOR14b) through real-time PCR.
  • - It finds that while the cold response is similar in seedlings and callus cultures, gene expression levels are lower in callus, and certain acids like phaseic acid and neophaseic acid increase significantly after cold acclimation.
  • - The research suggests that there is a light-independent cold-responsive mechanism in barley, with Dicamba enhancing overall gene expression and reactions to cold stress, making it a valuable model for studying cold acclimation in cereals. *

Article Abstract

In this study, data is presented how dark-grown, embryogenic barley callus cells respond to cold without any light-dependent, chloroplast-related mechanism, independently of the systemic signals. The expression of HvCBF9, HvCBF14, and HvCOR14b genes, members of one of the most important cold-inducible regulatory system, was measured by real-time PCR. Characteristic of the cold response was similar in the crowns of seedlings and in dark-grown callus cultures, however, gene expression levels were lower in calli. Endogenous concentration of auxins, abscisic acid, and salicylic acid did not change, but phaseic acid and neophaseic acid showed robust accumulation after cold acclimation. Freezing tolerance of the cultures was also higher after 7 days of cold-hardening. The results suggest the presence of a basal, light-independent, cold-responsive activation of the CBF-COR14b pathway in barley cultures. The effects of Dicamba, the exogenous auxin analog used for maintaining tissue cultures were also studied. Dicamba seems to be a general enhancer of the gene expression and physiological responses to cold stress, but has no specific effect on the activation. Our data along with previous findings show that this system might be a suitable model for studying certain basic cellular mechanisms involved in the cold acclimation process in cereals.

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Source
http://dx.doi.org/10.1007/s12033-012-9569-9DOI Listing

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