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CheA-receptor interaction sites in bacterial chemotaxis. | LitMetric

CheA-receptor interaction sites in bacterial chemotaxis.

J Mol Biol

Department of Chemistry and Biochemistry, University of California Santa Barbara, Santa Barbara, CA 93106–9510, USA.

Published: September 2012

AI Article Synopsis

  • Bacterial chemotaxis involves transmembrane chemoreceptors, CheA kinase, and CheW proteins working together to help bacteria swim towards favorable environments.
  • This study focuses on the interaction between CheA and chemoreceptors in Thermotoga maritima, using NMR spectroscopy, and also examines similar interactions in Escherichia coli.
  • Findings reveal that CheA binds to receptors like CheW does, indicating that changes in CheA's shape are essential for forming a complex with CheW and chemoreceptors, which is crucial for CheA's activation.

Article Abstract

In bacterial chemotaxis, transmembrane chemoreceptors, the CheA histidine kinase, and the CheW coupling protein assemble into signaling complexes that allow bacteria to modulate their swimming behavior in response to environmental stimuli. Among the protein-protein interactions in the ternary complex, CheA-CheW and CheW-receptor interactions were studied previously, whereas CheA-receptor interaction has been less investigated. Here, we characterize the CheA-receptor interaction in Thermotoga maritima by NMR spectroscopy and validate the identified receptor binding site of CheA in Escherichia coli chemotaxis. We find that CheA interacts with a chemoreceptor in a manner similar to that of CheW, and the receptor binding site of CheA's regulatory domain is homologous to that of CheW. Collectively, the receptor binding sites in the CheA-CheW complex suggest that conformational changes in CheA are required for assembly of the CheA-CheW-receptor ternary complex and CheA activation.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3418421PMC
http://dx.doi.org/10.1016/j.jmb.2012.05.023DOI Listing

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