AI Article Synopsis
- A new high-resolution melt assay has been developed to effectively differentiate between the live vaccine strain 1B of Chlamydia abortus and various field strains.
- This technique is based on specific single nucleotide polymorphisms that allow for clear identification.
- The assay is cost-effective, user-friendly, and quicker to perform compared to traditional PCR-RFLP methods.
Article Abstract
We describe a novel high-resolution melt assay that clearly differentiates Chlamydia abortus live vaccine strain 1B from field C. abortus strains and field wild-type isolates based on previously described single nucleotide polymorphisms. This modern genotyping technique is inexpensive, easy to use, and less time-consuming than PCR-RFLP.
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| http://dx.doi.org/10.1016/j.mimet.2012.05.014 | DOI Listing |
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