Institute for Veterinary Medical Research, Centre for Agricultural Research, Hungarian Academy of Sciences, PO Box 18, H-1581 Budapest, Hungary.
Published: July 2012
AI Article Synopsis
Article Abstract
A rapid, easy method involving a polymerase chain reaction (PCR) assay followed by restriction fragment length polymorphism (RFLP) analysis was developed to differentiate the 2 phylogenetic lineages of Pasteurella multocida. The PCR targeted the 16S ribosomal RNA gene, and the RFLP involved separate digestions with HindIII, EarI, and MlsI. The method was applied to 16 isolates of P. multocida from different hosts and the isolates were clearly assigned to 1 of the 2 lineages. The phylogenetic lineages did not match with the phenotypic-based identification at the subspecies level.
Fleas are primarily parasites of small mammals and serve as essential vectors of the transmission of plague. The subfamily Amphipsyllinae (Siphonaptera: Leptopsyllidae) consists of 182 species across 13 genera, widely distributed worldwide. Only two species of Amphipsyllinae have been sequenced for complete mitogenomes to date.
Background: Pertussis is a highly contagious respiratory disease caused by (BP). Despite global control of pertussis cases through the Expanded Programme on Immunization (EPI), there has been a significant increase in the incidence of pertussis in recent years, characterized by a "resurgence" in developed countries with high immunization rates as well as a comparable reemergence in certain areas of China. We aim to explore the genotypes and antimicrobial susceptibility of circulating BP from children in Hebei.
Botswana, like the rest of the world, has been significantly impacted by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In December 2022, we detected a monophyletic cluster of genomes comprising a sublineage of the Omicron variant of concern (VOC) designated as B.1.
Mussels and tubeworms have evolved similar adhesive systems to cope with the hydrodynamics of intertidal environments. Both secrete adhesive proteins rich in DOPA, a post-translationally modified amino acid playing essential roles in their permanent adhesion. DOPA is produced by the hydroxylation of tyrosine residues by tyrosinase enzymes, which can also oxidize it further into dopaquinone.
In this survey, 14 populations of were collected from the rhizosphere of eight fruit and nut trees in Fars province, Southern Iran. The phylogenetic relationships of these populations with other representatives of the species were investigated using sequences of cytochrome c oxidase subunit 1 mitochondrial gene () and D2-D3 expansion fragments of 28S rDNA. Phylogenetic studies indicated a close relationship of the currently sequenced populations with known haplotype groups (HG) in the tree and revealed two separate lineages in the 28S rDNA tree.
