AI Article Synopsis
- The proposed method utilizes next-generation sequencing to quickly identify causal alleles in genetically modified organisms, making it more efficient than current techniques.
- Unlike traditional approaches that require recovering homozygous lines or multiple backcrosses, this method streamlines the process using SNP-ratio mapping.
- This technique is particularly valuable for targeting lethal mutations and modifier genes within complicated genetic or transgenic backgrounds.
Article Abstract
We present a generally applicable method allowing rapid identification of causal alleles in mutagenized genomes by next-generation sequencing. Currently used approaches rely on recovering homozygotes or extensive backcrossing. In contrast, SNP-ratio mapping allows rapid cloning of lethal and/or poorly transmitted mutations and second-site modifiers, which are often in complex genetic/transgenic backgrounds.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416015 | PMC |
| http://dx.doi.org/10.1534/genetics.112.141341 | DOI Listing |
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