Infrared (IR) wavelengths penetrate skin and can selectively image volumes of subsurface blood. Twenty-eight blunt force injuries on nine decedents were photographed with color and IR film to compare the ability of each to image the injury. Of the 28 injuries, 10 were clinically interpreted as contusions, 4 as abrasions, 10 as abraded contusions, and 4 as erythematous discolorations, nos. Twenty-four of these injuries were incised to determine the presence and extent of subsurface bleeding. It was found that contusion had been clinically missed in five cases, three because they were hidden by abrasion and two because they appeared only as a mild cutaneous erythema. All five cases were detected by IR and verified by incision. Melanin pigmentation contributed to masking of contusion in one case and postmortem lividity confounded interpretation of contusion in one case. In all 14 injuries featuring abrasion as a component, IR de-emphasized or eliminated the visibility of abrasion. A single false negative was reported in which a visible contusion, verified by incision, was not seen with IR. In comparing injury patterns, color and IR renditions were dissimilar in a number of cases reflecting the difference between the manner in which light and IR photography imaged the depth and volume of blood within an injury. In summary, IR imaging of wounds can discover hidden bruises, verify clinical diagnosis of bruises, and augment interpretation of wound patterns.
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JBJS Essent Surg Tech
January 2025
Department of Neurosurgery, Center for Neuroscience and Spine, Virginia Mason Medical Center, Seattle, Washington.
Background: Prone transpsoas lumbar interbody fusion (PTP) is a newer technique to treat various spinal disc pathologies. PTP is a variation of lateral lumbar interbody fusion (LLIF) that is performed with the patient prone rather than in the lateral decubitus position. This approach offers similar benefits of lateral spinal surgery, which include less blood loss, shorter hospital stay, and quicker recovery compared with traditional open spine surgery.
View Article and Find Full Text PDFMicron
December 2024
School of Sciences, European University Cyprus, Nicosia 2404, Cyprus. Electronic address:
Atomic Force Microscopy (AFM) nanoindentation is the most effective method for determining the mechanical properties of soft biological materials and biomaterials at the nanoscale, with significant applications in many areas, including cancer diagnosis. However, a major drawback of this method is the complexity of the experimental procedure and data processing, which requires several calibration steps.To avoid this complexity, the AFM tip is usually approximated as a perfect cone.
View Article and Find Full Text PDFInt J Mol Sci
December 2024
Department of Anesthesiology & Perioperative Medicine, Mayo Clinic, Rochester, MN 55905, USA.
Aging disrupts multiple homeostatic processes, including autophagy, a cellular process for the recycling and degradation of defective cytoplasmic structures. Acute treatment with the autophagy inhibitor chloroquine blunts the maximal forces generated by the diaphragm muscle, but the mechanisms underlying neuromuscular dysfunction in old age remain poorly understood. We hypothesized that chloroquine treatment increases the presynaptic retention of the styryl dye FM 4-64 following high-frequency nerve stimulation, consistent with the accumulation of unprocessed bulk endosomes.
View Article and Find Full Text PDFLow Urin Tract Symptoms
January 2025
Farwaniya Hospital, Farwaniya, Kuwait.
Objectives: During holmium laser enucleation of the prostate (HOLEP), blunt dissection (BD) by pushing the tip of the scope may exert mechanical force on the sphincter that could be avoided by adopting laser dissection (LD). This study evaluates the continence recovery in consecutive patients who underwent BD and LD.
Methods: A prospective cohort of patients who underwent LD was compared with a retrospective control that underwent BD.
J Physiol
January 2025
Aging and Metabolism Research Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA.
We previously reported that elevated expression of phospholipid hydroperoxide glutathione peroxidase 4, an enzyme that regulates membrane lipid hydroperoxides, can mitigate sarcopenia in mice. However, it is still unknown whether a pharmacological intervention designed to modulate lipid hydroperoxides might be an effective strategy to reduce sarcopenia in aged mice. Here we asked whether a newly developed compound, CMD-35647 (CMD), can reduce muscle atrophy induced by sciatic nerve transection.
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