The Taylor Spatial Frame (TSF) is a circular external fixator used to treat complex fractures and skeletal deformities. The device consists of 2 rings attached to bone by wires or half pins, connected by universal hinge joints to 6 independent telescopic struts, creating a hexapod. The output piece is defined as the movable ring, which has 6 degree of freedom relative to the other ring, which is the base. With 6 degree of freedom, the movable platform is capable of moving in 3 linear directions and 3 angular directions singularly or in any combination. These hexapod devices require complex mathematical software programs to accurately control the output piece. In the case of the TSF, the deformity and positional frame parameters can be indentified on postoperative radiographs. They are then input into internet-based software to calculate strut adjustments required to achieve deformity correction. When treating fractures with the TSF, the rings can be connected using FastFx struts. These struts can be locked in position or left in a sliding mode (unlocked) allowing manual manipulation of the frame to acutely correct the position of the fracture fragments. This reduction is rarely perfect however and often requires further postoperative frame adjustments using software calculations. We describe an intra-operative method for accurately adjusting the frame in its locked mode without the need for software input.
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Single-omics approaches often provide a limited view of complex biological systems, whereas multiomics integration offers a more comprehensive understanding by combining diverse data views. However, integrating heterogeneous data types and interpreting the intricate relationships between biological features-both within and across different data views-remains a bottleneck. To address these challenges, we introduce COSIME (Cooperative Multi-view Integration and Scalable Interpretable Model Explainer).
View Article and Find Full Text PDFMatrix Biol
January 2025
Department of Pharmacology & Immunology, Proteomics Center, Medical University of South Carolina, Charleston, SC. Electronic address:
Collagen stroma interactions within the extracellular microenvironment of breast tissue play a significant role in breast cancer, including risk, progression, and outcomes. Hydroxylation of proline (HYP) is a common post-translational modification directly linked to breast cancer survival and progression. Changes in HYP status lead to alterations in epithelial cell signaling, extracellular matrix remodeling, and immune cell recruitment.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
January 2025
Department of Chemical Engineering, Stanford University, Stanford, CA 94305.
The crowded bacterial cytoplasm is composed of biomolecules that span several orders of magnitude in size and electrical charge. This complexity has been proposed as the source of the rich spatial organization and apparent anomalous diffusion of intracellular components, although this has not been tested directly. Here, we use biplane microscopy to track the 3D motion of self-assembled bacterial genetically encoded multimeric nanoparticles (bGEMs) with tunable size (20 to 50 nm) and charge (-3,240 to +2,700 e) in live cells.
View Article and Find Full Text PDFJ Neurosci
January 2025
Department of Neurobiology and Behavior and Center for the Neurobiology of Learning and Memory, University of California, Irvine, Irvine, California 92697 USA
Cerebral amyloid-beta (Aβ) accumulation, a hallmark pathology of Alzheimer's disease (AD), precedes clinical impairment by two to three decades. However, it is unclear whether Aβ contributes to subtle memory deficits observed during the preclinical stage. The heterogenous emergence of Aβ deposition may selectively impact certain memory domains, which rely on distinct underlying neural circuits.
View Article and Find Full Text PDFAdv Sci (Weinh)
January 2025
Division of Endocrinology, Metabolism and Lipid Research, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8127, St. Louis, MO, 63110, USA.
Glioblastoma (GBM) is an aggressive form of brain cancer that is highly resistant to therapy due to significant intra-tumoral heterogeneity. The lack of robust in vitro models to study early tumor progression has hindered the development of effective therapies. Here, this study develops engineered GBM organoids (eGBOs) harboring GBM subtype-specific oncogenic mutations to investigate the underlying transcriptional regulation of tumor progression.
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