The analysis of different selenium compounds toxicity and level of selenium intake in different countries are given in the article. It showed that population of Russian Federation have not defined selenodeficiency. Thus there is no need in wide-ranging fortification of foodstuff by selenium. For increasing of selenium level in particularized foodstuffs (biology activity supplements, specialized foodstuff for pregnant, children, dietetic, medicinal and prophylactic foodstuff) are preferred the organic forms of selenium. Inorganic forms of selenium (selenites and selenates) could use only in the composition of biology activity supplements. However in this case the organic form is more preferably too.
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Trends Biotechnol
March 2025
Tidetron Bioworks Technology (Guangzhou) Co., Ltd, Guangzhou Qianxiang Bioworks Co., Ltd, Guangzhou, Guangdong 510000, PR China. Electronic address:
Targeted random mutagenesis is crucial for breeding, directed evolution, and gene function studies, yet efficient tools remain scarce. Here, we present obligate mobile element guided activity (OMEGA)-R, an innovative targeted random mutagenesis system that integrates SpyCatcher-enIscB and PolI3M-TBD-SpyTag, outperforming existing state-of-the-art technologies in key metrics, such as protein size, mutagenesis efficiency, window length, and continuity. OMEGA-R achieves a dramatic enhancement of on-target mutagenesis, reaching a rate of 1.
View Article and Find Full Text PDFAnal Chim Acta
May 2025
State Key Laboratory of Natural Medicines, China Pharmaceutical University, No. 639 Longmian Dadao, Nanjing, 211198, China. Electronic address:
Background: Traditional studies of protein responses to external stimuli primarily focus on changes in protein abundance, often overlooking the critical role of protein conformational alterations. To address this gap, we developed Protein Abundance and Conformation Analysis (PACA), an integrative method that quantifies both protein abundance and conformational changes. PACA combines conventional quantitative proteomics for abundance measurements with Target Response Accessibility Profiling (TRAP), a technique that captures conformational changes in situ by applying reductive dimethylation to label accessible lysine residues in living cells before lysis.
View Article and Find Full Text PDFJ Genet Eng Biotechnol
March 2025
Zoology Department, Faculty of Science, Al-Azhar University, 71524 Assiut, Egypt.
Due to bleomycin's cytotoxic characteristics, which include cardiotoxicity, this investigation looked at the effectiveness of costus ethanolic extract in reducing cardiotoxicity in male rats receiving bleomycin therapy. Forty adult male rats (160-200 g) were evenly allocated into four groups: group (1) included normal rats serving as the control; group (2) included normal rats administered 200 mg/kg of costus ethanolic extract (CEE) orally for 6 weeks; group (3) consisted of rats receiving bleomycin (15 mg/kg twice weekly, ip) for 6 weeks; and group (4) involved rats treated orally with CEE (200 mg/kg/day) for 6 weeks following bleomycin intoxication. The results indicated that the CEE significantly reversed the cardiological deteriorations brought on by bleomycin; this was demonstrated by a considerable increase in cardiac SOD, GPx, GSH, and CAT, along with a substantial decrease in cardiac MDA, NO, and DNA fragmentation.
View Article and Find Full Text PDFJ Genet Eng Biotechnol
March 2025
Faculty of Biotechnology and Genetic Engineering, Sylhet Agricultural University, Sylhet 3100, Bangladesh; Department of Molecular Biology and Genetic Engineering, Sylhet Agricultural University, Sylhet 3100, Bangladesh. Electronic address:
One of the largest and most significant transcription factor gene families in plants is the SQUAMOSA promoter binding protein (SBP) gene family and they perform critical regulatory roles in floral enhancement, fruit development, and stress resistance. The SBP protein family (also known as SPL) has not yet been thoroughly studied in the staple fruit crop, banana. A perennial monocot plant, banana is essential for ensuring food and nutrition security.
View Article and Find Full Text PDFJ Genet Eng Biotechnol
March 2025
Department of Bioinformatics, University of North Bengal, District-Darjeeling, West Bengal 734013, India. Electronic address:
Background: Acquired Immunodeficiency Syndrome (AIDS) is a critical global health issue caused by the human immunodeficiency virus (HIV). It has different strains and subtypes; among these, Subtype C accounts for higher infection rates than others. Despite its high prevalence, the molecular interactions with host receptors, specifically CD4, have not yet been explored.
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