Plant microRNAs have a vital role in various abiotic stress responses by regulating gene expression. Heat stress is one of the most severe abiotic stresses, and affects plant growth and development, even leading to death. To identify heat-responsive miRNAs at the genome-wide level in Populus, Solexa sequencing was employed to sequence two libraries from Populus tomentosa, treated and untreated by heat stress. Sequence analysis identified 134 conserved miRNAs belonging to 30 miRNA families, and 16 novel miRNAs belonging to 14 families. Among these miRNAs, 52 miRNAs from 15 families were responsive to heat stress and most of them were down-regulated. qRT-PCR analysis confirmed that the conserved and novel miRNAs were expressed in P. tomentosa, and revealed similar expression trends to the Solexa sequencing results obtained under heat stress. One hundred and nine targets of the novel miRNAs were predicted. This study opens up a new avenue for understanding the regulatory mechanisms of miRNAs involvement in the heat stress response of trees.
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http://dx.doi.org/10.1016/j.gene.2012.05.034 | DOI Listing |
Sci Rep
January 2025
School of Instrumental Science and Engineering, Harbin Institute of Technology, 150001, Harbin, People's Republic of China.
In this study, a method for predicting the thermal shock life of coatings is proposed, and a model for predicting the thermal shock life of coatings based on high temperature thermal shock life test and three-dimensional heat transfer analysis is established. Firstly, the thermal shock life of coatings at different cooling and heating cycle temperatures is obtained through a designed thermal shock life testing device for silicide coatings at a wide-temperature range from 500℃ to 3000℃. Secondly, the actual thickness of the coating and the continuous oxidation in the thermal shock life test are taken into consideration.
View Article and Find Full Text PDFBiochem Pharmacol
January 2025
Shanghai Frontiers Science Center of Drug Target Identification and Delivery, School of Pharmaceutical Sciences, Shanghai Jiao Tong University, Shanghai 200240 PR China; National Key Laboratory of Innovative Immunotherapy, Shanghai Jiao Tong University, Shanghai 200240 PR China. Electronic address:
Multiple organ dysfunction syndrome (MODS) is the major cause of mortality of patients in intensive care units. The elusive mechanisms of tissue damage in MODS and limited therapeutic options encourage us to seek effective therapies to MODS. PANoptosis has recently been proven to be the key player in both heat stress and sepsis-mediated MODS.
View Article and Find Full Text PDFComput Biol Chem
January 2025
Department of Otolaryngology, Shenzhen Traditional Chinese Medicine Hospital, Shenzhen 518033, China. Electronic address:
The pathophysiological distinctions between osteoarthritis (OA) and diabetic osteoarthritis (DOA) are critical yet not well delineated. In this study, we employed single-cell RNA sequencing to clarify the unique cellular and molecular mechanisms underpinning the progression of both conditions. We identified a novel subpopulation of chondrocytes in DOA, termed 'Heat Shock' chondrocytes, marked by the expression of distinct molecular markers including HSPA1A, HSPA1B, HSPB1, and HSPA8.
View Article and Find Full Text PDFSci Total Environ
January 2025
Cawthron Institute, 98 Halifax Street East, Nelson 7010, New Zealand. Electronic address:
In New Zealand, the frequency and intensity of marine heatwaves (MHWs) and blooms of the harmful algal species, Alexandrium pacificum, are increasing in areas where there are natural reefs and commercial farms of the mussel, Perna canaliculus. In this study, we assessed the whole organism, tissue and molecular-level response of juvenile (spat) P. canaliculus exposed to these abiotic and biotic stressors, alone and together.
View Article and Find Full Text PDFTrop Anim Health Prod
January 2025
School of Molecular Diagnostics, Prophylaxis, and Nanobiotechnology, ICAR- Indian Institute of Agricultural Biotechnology, Garkhtanga, Ranchi, 834003, Jharkhand, India.
Climate change poses significant challenges to livestock production worldwide. Wherein, it affects communities in developing nations primarily dependent on agriculture and animal husbandry. Its direct and indirect deleterious effects on agriculture and animal husbandry includes aberrant changes in weather patterns resulting in disturbed homeorhetic mechanism of livestock vis a vis indirectly affecting nutrient composition of feed and fodder.
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