AI Article Synopsis
- Chitosan oligosaccharides (COS) exhibit various biological activities, including antioxidant and anti-inflammatory effects.
- In a study on LPS-induced N9 microglial cells, COS pretreatment significantly reduced nitric oxide (NO) production by inhibiting the expression of inducible nitric oxide synthase (iNOS).
- COS also prevented the activation of key signaling pathways such as p38 MAPK, ERK1/2, and the nuclear factor-κB (NF-κB) pathway, suggesting its potential role in modulating inflammatory responses.
Article Abstract
Chitosan oligosaccharides (COS) have been reported to exert many biological activities, such as antioxidant, antitumor and anti-inflammatory effects. In the present study, we examined the effect of COS on nitric oxide (NO) production in LPS induced N9 microglial cells. Pretreatment with COS (50~200 μg/ml) could markedly inhibit NO production by suppressing inducible nitric oxide synthase (iNOS) expression in activated microglial cells. Signal transduction studies showed that COS remarkably inhibited LPS-induced phosphorylation of p38 MAPK and ERK1/2. COS pretreatment could also inhibit the activation of both nuclear factor-κB (NF-κB) and activator protein-1 (AP-1). In conclusion, our results suggest that COS could suppress the production of NO in LPS-induced N9 microglial cells, mediated by p38 MAPK and ERK1/2 pathways.
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| http://dx.doi.org/10.1007/s10719-012-9392-3 | DOI Listing |
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