To screen aerobic heterotrophic denitrifying bacteria from various samples and detect metabolites quantitatively during their denitrification process. The bacteria were screened by limit dilution and crossed separation. Nitrate and nitrite were determined using phenol disulfonic acid and N-(1-naphthyl)-ethylene diamine by ultraviolet spectrophotometry. The N2 was detected by gas chromatography. Six aerobic heterotrophic bacteria were isolated and YY-5 was demonstrated to be the greatest denitrifer. The degradation rate of its hydroxylamine oxidoreductase can reached to 70.9%. Above all, there is a good balance between the removal of total nitrogen and production of N2 in process. No N2O was produced and no nitrate or nitrite were accumulated. The high ability of aerobic heterotrophic bacterium YY-5 for denitrification was screened, which showed higher homology with Acinetobacter genus through 16S rRNA blast.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
Enrichment of a heterotrophic nitrifying and aerobic denitrifying bacterial consortium: Microbial community succession and nitrogen removal characteristics and mechanisms.
Bioresour Technol
December 2024
Department of Ecology, College of Life Science and Technology, Jinan University, Guangzhou 510632, Guangdong, China. Electronic address:
This study cultivated a bacterial consortium (S60) from landfill leachate that exhibited effective heterotrophic nitrification and aerobic denitrification (HN-AD) properties. Under aerobic conditions, the removal of NH-N reached 100 % when the S60 consortium utilised NH-N either as the sole nitrogen source or in combination with NO-N and NO-N. Optimal HN-AD performance was achieved with sodium acetate as a carbon source and a pH of 7.
View Article and Find Full Text PDFEffective organic matter removal via bio-adsorption prior to anammox process and utilization of carbon-rich sludge.
J Environ Manage
December 2024
Key Laboratory of Urban Storm Water System and Water Environment Ministry of Education, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China; Sino-Dutch R&D Centre for Future Wastewater Treatment Technologies/Key Laboratory of Urban Stormwater System and Water Environment, Beijing University of Civil Engineering and Architecture, Beijing, 100044, China.
Excessive organic matter in the anaerobic ammonia oxidation (Anammox) leads to the growth of a large number of heterotrophic bacteria, which disrupts the anaerobic ammonia oxidation. The adsorption-anaerobic ammonia oxidation process can effectively reduce excessive organic matter, capturing it instead of consuming it, which is a sustainable development technology. In this study, utilizing the excellent adsorption performance of aerobic granular sludge (AGS), an adsorption-regeneration process was employed to remove organic matter at the front end of the Anammox process through bio-adsorption in an artificial simulated domestic sewage environment, and it was successfully used for denitrification.
View Article and Find Full Text PDFSolids retention time modulates nutrient removal in pilot-scale anaerobic-aerobic-anoxic process: Carbon allocation patterns and microbial insights.
Water Res
December 2024
Key Laboratory of the Three Gorges Reservoir Region's Eco-Environment, Ministry of Education, Chongqing University, Chongqing 400045, China.
Anaerobic-aerobic-anoxic (AOA) process is a promising configuration to retrofit current wastewater treatment plants with intensified carbon utilization and nutrient removal, but lacks process optimization for scaling-up in real wastewater scenarios. Solids retention time (SRT) is a fundamental parameter of activated sludge process, but its roles in the AOA process remain vague. Here, we established a pilot-scale AOA process at different SRTs (10, 20, 30 d) to investigate the comprehensive responses and potential mechanisms.
View Article and Find Full Text PDFEffects of feeding and aeration strategies on NO production and emission by an aerobic granular sludge system for municipal wastewater treatment.
Chemosphere
December 2024
Department of Sanitary and Environmental Engineering, Federal University of Santa Catarina, Florianopolis, SC, 88040-900, Brazil.
The effects of a single feeding cycle followed by a continuous aeration phase (AND) and a step-feeding cycle followed by intermittent aerobic/idle phases (AND) on the production and emission of nitrous oxide (NO) from aerobic granular sludge (AGS) from real domestic sewage were studied. Higher NO emissions were observed in the AND treatment, and 9.2 ± 4.
View Article and Find Full Text PDFUnravelling depth layers of microbial communities, nitrogen transformation rate, and extracellular polymeric substances in anammox granules.
Bioresour Technol
November 2024
Key Laboratory of Agro-Forestry Environmental Processes and Ecological Regulation of Hainan Province, School of Environmental Science and Engineering, Hainan University, Haikou 570228, China. Electronic address:
Anammox granules harbor a variety of bacteria, with each granule layer providing a niche for bacteria with specific metabolic functions. To investigate microbial distribution, nitrogen transformation rates, and extracellular polymeric substance (EPS) content across the depth layers of anammox granules, granules sized 0.9-2.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!