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In situ Synthesis of Oligonucleotide Arrays on Surfaces Coated with Crosslinked Polymer Multilayers. | LitMetric

AI Article Synopsis

  • The study presents a method for creating oligonucleotide arrays on surfaces treated with crosslinked polymer layers using a process called layer-by-layer assembly, specifically employing branched polyethyleneimine and a special polymer, PVDMA.
  • After modifying the polymer-coated surfaces with certain chemicals, the researchers achieved oligonucleotide arrays that rivaled traditional silica glass in performance, with good fluorescence intensity and stability during repeated hybridization processes.
  • This innovative technique enables the production of flexible, robust oligonucleotide arrays on various materials, showcasing a significant advancement in the synthesis of complex molecules for potential applications in macromolecular synthesis and screening.

Article Abstract

We report an approach to the in situ synthesis of oligonucleotide arrays on surfaces coated with crosslinked polymer multilayers. Our approach makes use of methods for the 'reactive' layer-by-layer assembly of thin, amine-reactive multilayers using branched polyethyleneimine (PEI) and the azlactone-functionalized polymer poly(2-vinyl-4,4'-dimethylazlactone) (PVDMA). Post-fabrication treatment of film-coated glass substrates with d-glucamine or 4-amino-1-butanol yielded hydroxyl-functionalized films suitable for the Maskless Array Synthesis (MAS) of oligonucleotide arrays. Glucamine-functionalized films yielded arrays of oligonucleotides with fluorescence intensities and signal-to-noise ratios (after hybridization with fluorescently labeled complementary strands) comparable to those of arrays fabricated on conventional silanized glass substrates. These arrays could be exposed to multiple hybridization-dehybridization cycles with only moderate loss of hybridization density. The versatility of the layer-by-layer approach also permitted synthesis directly on thin sheets of film-coated poly(ethylene terephthalate) (PET) to yield flexible oligonucleotide arrays that could be readily manipulated (e.g., bent) and cut into smaller arrays. To our knowledge, this work presents the first use of polymer multilayers as a substrate for the multi-step synthesis of complex molecules. Our results demonstrate that these films are robust and able to withstand the ~450 individual chemical processing steps associated with MAS (as well as manipulations required to hybridize, image, and dehybridize the arrays) without large-scale cracking, peeling, or delamination of the thin films. The combination of layer-by-layer assembly and MAS provides a means of fabricating functional oligonucleotide arrays on a range of different materials and substrates. This approach may also prove useful for the fabrication of supports for the solid-phase synthesis and screening of other macromolecular or small-molecule agents.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3352262PMC
http://dx.doi.org/10.1021/cm202720qDOI Listing

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