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Expression of bacterial GshF in Pichia pastoris for glutathione production. | LitMetric

Expression of bacterial GshF in Pichia pastoris for glutathione production.

Appl Environ Microbiol

Institute of Microbiology, Chinese Academy of Sciences, Beijing, People's Republic of China.

Published: August 2012

Conventionally, two consecutive enzymatic reactions catalyzed by γ-glutamylcysteine synthetase and glutathione synthetase are most commonly used for glutathione production. Here we demonstrate that bacterial bifunctional GshF can be used for glutathione production in a eukaryotic system without accumulation of the intermediate γ-glutamylcysteine.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3416392PMC
http://dx.doi.org/10.1128/AEM.00509-12DOI Listing

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